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Early lineage segregation of multipotent embryonic mammary gland progenitors

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE109711 | GEO | 2018/06/04

REPOSITORIES: GEO

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Early lineage segregation of multipotent embryonic mammary gland progenitors.

Project description:The mammary gland is composed of basal cells and luminal cells. It is generally believed that the mammary gland arises from embryonic multipotent progenitors, but it remains unclear when lineage restriction occurs and what mechanisms are responsible for the switch from multipotency to unipotency during its morphogenesis. Here, we perform multicolour lineage tracing and assess the fate of single progenitors, and demonstrate the existence of a developmental switch from multipotency to unipotency during embryonic mammary gland development. Molecular profiling and single cell RNA-seq revealed that embryonic multipotent progenitors express a unique hybrid basal and luminal signature and the factors associated with the different lineages. Sustained p63 expression in embryonic multipotent progenitors promotes unipotent basal cell fate and was sufficient to reprogram adult luminal cells into basal cells by promoting an intermediate hybrid multipotent-like state. Altogether, this study identifies the timing and the mechanisms mediating early lineage segregation of multipotent progenitors during mammary gland development.

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Early lineage segregation of multipotent embryonic mammary gland progenitors [expression]

Project description:The mammary gland (MG) is composed of basal cells (BCs) and luminal cells (LCs). While it is generally believed that MG arises from embryonic multipotent progenitors (EMPs), it remains unclear when lineage restriction occurs and what are the mechanisms responsible for the switch from multipotency to unipotency during MG morphogenesis. Here, we performed multicolor lineage tracing and assessed the fate of single embryonic progenitors during mouse MG development. We demonstrated the existence of a developmental switch from multipotency to unipotency during embryonic MG development. Molecular profiling and single cell RNA-seq revealed the hybrid gene expression of EMPs, and the gene trajectory and lineage segregation occurring during MG development. In situ characterization showed that one of the earliest signs of lineage segregation consists in the restricted expression of p63 in the future BCs. Sustained p63 expression during MG development promotes unipotent BC fate in EMPs. Altogether, this study identifies the timing and the mechanisms mediating the switch from multipotency to unipotency during MG development. To understand the molecular mechanisms regulating multipotency during embryonic development, we isolated EMPs by FACS at E14, performed their transcriptional profiling by microarray and compared their transcriptome to adult BCs and LCs

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Early lineage segregation of multipotent embryonic mammary gland progenitors

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors

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Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-Seq II]

Project description:We report single cell RNA-seq data that reveals that Embryonic Multipotent Progenitors (EMPs) in the mammary gland express a unique hybrid basal and luminal signature associated with the different adult lineages.

2018-06-04 | GSE110351 | GEO

Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I]

Project description:The mammary gland (MG) is composed of basal cells (BCs) and luminal cells (LCs). While it is generally believed that MG arises from embryonic multipotent progenitors (EMPs), it remains unclear when lineage restriction occurs and what are the mechanisms responsible for the switch from multipotency to unipotency during MG morphogenesis. Here, we performed multicolor lineage tracing and assessed the fate of single progenitors and demonstrated the existence of a developmental switch from multipotency to unipotency during embryonic MG development. Molecular profiling and single cell RNA-seq revealed that EMPs express a unique hybrid basal and luminal signature and the factors associated with the different lineages. Sustained p63 expression in EMPs promotes unipotent BC fate and was sufficient to reprogram adult LCs into BCs by promoting an intermediate hybrid multipotent like state. Altogether, this study identifies the timing and the mechanisms mediating the early lineage segregation of multipotent progenitors during MG development.

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Early lineage segregation of multipotent embryonic mammary gland progenitors [expression]

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors [expression]

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Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-Seq II]

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-Seq II]

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Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I]

Project description:Early lineage segregation of multipotent embryonic mammary gland progenitors [RNA-seq I]

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Luminal progenitors restrict their lineage potential during mammary gland development.

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Dynamic analysis of gene expression and genome wide transcription factor binding during lineage-specification of multipotent progenitors

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

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