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Comparative transcriptomic analysis of Central Neurocytoma-derived tumor spheres as compared to its differentiated cells and fetal SVZ-derived neural stem cells.

ABSTRACT: We obtained tissues of Central neurocytomas (CNs) from surgeries of patients, and then we performed primary cultures of CN tissues. Tumor spheres of CN were isolated from the differentiated CN cells. Fetal subventricular zone tissues (this sample was derived from inevitable abortion because of fatal defects) were primary cultured as well. Going forward, we compared the RNA of the pools via microarray to identify markers of tumor stem cells of Central Neurocytomas (CNs) as compared to its differentiated cells and fetal subventricular zone (SVZ)-derived neural stem cells (NSCs) to be able to characterize its origin of CN. Interestingly, CN-tumor spheres have characteristics of Transit amplifying Type C cells and Radial glial cells (RGCs). Moreover CN-tumor spheres expressed well known cancer stem cells markers of glioblastomas.

ORGANISM(S): Homo sapiens

PROVIDER: GSE110066 | GEO | 2021/12/31

REPOSITORIES: GEO

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