Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE110354 | GEO | 2018/08/23

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Accurate Recycling of Parental Histones Reproduces the Histone Modification Landscape during DNA Replication.

Project description:Chromatin organization is disrupted genome-wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naive histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is faithfully copied during DNA replication or the epigenome is perturbed. Here we develop chromatin occupancy after replication (ChOR-seq) to determine histone PTM occupancy immediately after DNA replication and across the cell cycle. We show that H3K4me3, H3K36me3, H3K79me3, and H3K27me3 positional information is reproduced with high accuracy on newly synthesized DNA through histone recycling. Quantitative ChOR-seq reveals that de novo methylation to restore H3K4me3 and H3K27me3 levels occurs across the cell cycle with mark- and locus-specific kinetics. Collectively, this demonstrates that accurate parental histone recycling preserves positional information and allows PTM transmission to daughter cells while modification of new histones gives rise to complex epigenome fluctuations across the cell cycle that could underlie cell-to-cell heterogeneity.

| S-EPMC6202308 | biostudies-literature

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication

Project description:Chromatin organisation is disrupted genome wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naïve histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is copied during DNA replication or the epigenomeis perturbed. Here we develop Chromatin Occupancy after Replication, ChOR-seq, a technology that combines chromatin immunopreciptation of histone marks and purification of newly replicated DNA by streptavidin pull-down followed by next generation sequencing. We use this technology to address propagation of epigenetic states across cell division and reveal that accurate recycling of modified parental histones ensures that positional information of histone marks is faithfully reproduced on daughter strands and inherited to daughter cells.

2018-08-23 | GSE110352 | GEO

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication III

2018-08-23 | GSE116310 | GEO

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication II

Project description:Chromatin organisation is disrupted genome wide during DNA replication. On newly synthesized DNA, nucleosomes are assembled from new naïve histones and old modified histones. It remains unknown whether the landscape of histone post-translational modifications (PTMs) is copied during DNA replication or the epigenomeis perturbed. Here we develop Chromatin Occupancy after Replication, ChOR-seq, a technology that combines chromatin immunopreciptation of histone marks and purification of newly replicated DNA by streptavidin pull down followed by next generation sequencing. We use this technology to address propagation of epigenetic states across cell division and reveal that accurate recycling of modified parental histones ensures that positional information of histone marks is faithfully reproduced on daughter strands and inherited to daughter cells.

2018-08-23 | GSE110353 | GEO

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication

Project description:Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication

| PRJNA433528 | ENA

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication

Project description:Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication

| PRJNA433521 | ENA

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication III

Project description:Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication III

| PRJNA478190 | ENA

Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication II

Project description:Accurate recycling of parental histones reproduces the histone modification landscape during DNA replication II

| PRJNA433529 | ENA

Single-molecule imaging reveals control of parental histone recycling by free histones during DNA replication.

Project description:During replication, nucleosomes are disrupted ahead of the replication fork, followed by their reassembly on daughter strands from the pool of recycled parental and new histones. However, because no previous studies have managed to capture the moment that replication forks encounter nucleosomes, the mechanism of recycling has remained unclear. Here, through real-time single-molecule visualization of replication fork progression in Xenopus egg extracts, we determine explicitly the outcome of fork collisions with nucleosomes. Most of the parental histones are evicted from the DNA, with histone recycling, nucleosome sliding, and replication fork stalling also occurring but at lower frequencies. Critically, we find that local histone recycling becomes dominant upon depletion of endogenous histones from extracts, revealing that free histone concentration is a key modulator of parental histone dynamics at the replication fork. The mechanistic details revealed by these studies have major implications for our understanding of epigenetic inheritance.

| S-EPMC7500940 | biostudies-literature