Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Small RNA profiling of WT E14, Dgcr8_KO, Drosha_KO, Dicer_KO and Ago1&2_Double_KO mouse embryonic stem cells

ABSTRACT: Purpose: The goals of this study was to obtain the profile of miRNAs and endogeneous siRNAs of RNAi mutant mESCs

ORGANISM(S): Mus musculus

PROVIDER: GSE110945 | GEO | 2022/02/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Small RNA profiling of WT E14, Ago2_KO and Ago2_KO-AGO1 mouse embryonic stem cells.

Project description:Purpose: The goals of this study was to obtain the profile total miRNAs in WT E14, Ago2_KO mESCs to compare with Ago2_KO-AGO1 overexpressing AGO1.

2018-02-02 | GSE80415 | GEO

Transcriptome of AGO1&2 double KO mouse embryonic stem cells

Project description:Purpose: The goals of this study was to obtain the trasncriptome of WT mESCs to compare it with the transcriptome of all RNAi mutant mESCs (deposit separately).

2022-02-01 | GSE110942 | GEO

Small RNA profiling of WT E14, Ago2_KO and Ago1_KO mouse embryonic stem cells before and after XEN conversion and small RNA profiling after Immunoprecipitation of AGO1&2 proteins in WT E14, Ago1_KO and Ago2_KO mESCs.

Project description:Purpose: The goals of this study was to obtain the profile total miRNAs in WT E14, Ago2_KO and Ago1_KO mESCs to compare it with Immunoprecipitated microRNAs in AGO1&2 proteins.

2018-02-02 | GSE80211 | GEO

Small RNA profiling of Dicer_KO mouse embryonic stem cells.

Project description:Purpose: The goals of this study was to obtain the small RNA profiling of Dicer_KO mESCs.

2019-01-15 | GSE116452 | GEO

Transcriptome of WT mouse embryonic stem cells

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study was to obtain the trasncriptome of WT mESCs to compare it with the transcriptome of RNAi mutant mESCs (deposit separately).

2017-03-07 | GSE78971 | GEO

Transcriptome of new Dicer_KO mouse embryonic stem cells generated by paired CRISPR/Cas9 approach

Project description:Purpose: Next-generation sequencing (NGS) has revolutionized systems-based analysis of cellular pathways. The goals of this study was to obtain the trasncriptome of Dicer_KO mESCs to compare it with the transcriptome of WT mESCs (deposit separately).

2017-03-07 | GSE78973 | GEO

Ribosome profiling of WT E14, Dgcr8_KO, Dorsha_KO, Dicer_KO and Ago1&2_Double_KO mouse embryonic stem cells

Project description:miRNAs are short regulatory single stranded RNA sequences that upon complementary binding to mRNAs lead to the inhibition or degradation of their targets. This regulatory mechanisms has been shown to play crucial roles throughout the whole life cycle of animals and plants as well as in disease. While a plethora of methods exist to predict targets of miRNA, which suggest that up to 80% of the genome is miRNA regulated, it has recently been reported that many of these predictions are false positives, cell type specific or represent non-functional binding. In order to identify the subset of real functional miRNAs and their targets, we established miRNA pathway mutants in mouse embryonic stem cells (mESC), allowing the dissection of canonical and non-canonical functions of pathway members. Additional data integration of downstream regulatory layers (CLIP-seq, ribosome profiling and MS) enabled us to follow and track down real functional miRNA-gene interactions, which reduced the miRNA genome regulation to approximately 1%.

2022-08-08 | GSE135577 | GEO

Transcriptome of WT, Dgcr8_KO, Drosha_KO, and Ago1&2_double KO mouse embryonic stem cells cultured in 2i

Project description:Purpose: The goals of this study was to assess the impact of different RNAi-mutant proteins on mESC transcriptome in 2i condition.

2022-02-01 | GSE110941 | GEO

Transcriptome of new DROSHA_KO mouse embryonic stem cells generated by paired CRISPR/Cas9 approach

Project description:Purpose: The goals of this study was to obtain the trasncriptome of DROSHA_KO mESCs to compare it with the transcriptome of WT mESCs (deposit separately).

2020-01-01 | GSE103678 | GEO

Transcriptome of new DROSHA_KO mouse embryonic stem cells generated by paired CRISPR/Cas9 approach

Project description:Purpose: The goals of this study was to obtain the trasncriptome of DROSHA_KO mESCs to compare it with the transcriptome of WT mESCs (originally deposited in GSE78971 and included here).

2021-11-14 | GSE122627 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data