Transcriptomics

Dataset Information

0

ISR target genes in the liver of mock-injected and AP20187-treated mice of wildtype and Atf4-/- genotype


ABSTRACT: The molecular mechanisms linking the stress of unfolded proteins in the endoplasmic reticulum (ER stress) to glucose intolerance in obese animals are poorly understood. In this study enforced expression of a translation initiation 2α (eIF2α)-specific phosphatase, GADD34, was used to selectively compromise signaling in the eIF2(αP)-dependent arm of the ER unfolded protein response in liver of transgenic mice. The transgene resulted in lower liver glycogen levels and susceptibility to fasting hypoglycemia in lean mice and glucose tolerance and diminished hepato-steatosis in animals fed a high fat diet. Attenuated eIF2(αP) correlated with lower expression of the adipogenic nuclear receptor PPARγ and its upstream regulators, the transcription factors C/EBPα and C/EBPβ, in transgenic mouse liver, whereas eIF2α phosphorylation promoted C/EBP translation in cultured cells and primary hepatocytes. These observations suggest that eIF2(αP)-mediated translation of key hepatic transcriptional regulators of intermediary metabolism contributes to the detrimental consequences of nutrient excess. Keywords: genotype comparison

ORGANISM(S): Mus musculus

PROVIDER: GSE11116 | GEO | 2011/12/23

SECONDARY ACCESSION(S): PRJNA107011

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2011-12-23 | E-GEOD-11116 | biostudies-arrayexpress
2011-12-23 | GSE11210 | GEO
2011-12-23 | E-GEOD-11210 | biostudies-arrayexpress
2023-11-22 | GSE248013 | GEO
2017-08-24 | GSE102946 | GEO
2012-01-20 | E-GEOD-29929 | biostudies-arrayexpress
2015-10-01 | GSE69800 | GEO
2012-01-20 | GSE29929 | GEO
2017-08-27 | GSE60868 | GEO
2010-12-31 | E-GEOD-18115 | biostudies-arrayexpress