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Expression data from single cells from mouse primordial germ cell lineage (E6.25-E8.25, wild type and Blimp1KO)

ABSTRACT: Specification of germ cell fate is fundamental in development. With a highly representative single-cell microarray and rigorous quantitative-PCR analysis, we defined the genome-wide transcription dynamics that create primordial germ cells (PGCs) from the epiblast, a process that exclusively segregates them from their somatic neighbors. We also analyzed the effect of the loss of Blimp1, a key transcriptional regulator, on these dynamics. Our analysis revealed that PGC specification involves complex, yet highly ordered regulation of a large number of genes, proceeding under the strong influence of mesoderm induction with active repression of specific programs such as epithelial-mesenchymal transition, Hox gene activation, cell-cycle progression and DNA methyltransferase machinery. Remarkably, Blimp1 is essential for repressing nearly all the genes normally down-regulated in PGCs relative to their somatic neighbors, whereas it is dispensable for the activation of approximately half of the genes up-regulated in PGCs. Keywords: single cell analysis, time course, Blimp1 knocked out

ORGANISM(S): Mus musculus

PROVIDER: GSE11128 | GEO | 2008/08/22

SECONDARY ACCESSION(S): PRJNA106911

REPOSITORIES: GEO

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Transcription profiling of mouse single cells from primordial germ cell lineage (E6.25-E8.25, wild type and Blimp1KO)

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