Transcriptome-wide analysis of RNA binding and splicing alterations induced by SRSF2 P95 mutations in myelodysplasia
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ABSTRACT: Mutations in the splicing factor Serine Arginine Rich Splicing Factor 2 (SRSF2) occur in over 30% of patients with myelodysplasia (MDS) and a subset of patients with acute myeloid leukemia (AML) and portend a poor prognosis. SRSF2 is ubiquitously expressed and directs exon inclusion or exclusion in a sequence and context dependent manner. SRSF2 mutations almost exclusively affect the proline at position 95 in the C-terminus of the RNA binding domain. We here show how a mutation in SRSF2 (P95H) affects its normal function in vivo on a transcriptome-wide basis via RNA crosslinking and immunoprecipitation (HITS-CLIP) and parallel quantification of transcript levels (RNA-seq), leading to the identification of differentially bound and alternatively spliced SRSF2 targets. Keywords: Splicing, myeloid neoplasia, SRSF2, HITS-CLIP
ORGANISM(S): Homo sapiens
PROVIDER: GSE111900 | GEO | 2018/06/18
REPOSITORIES: GEO
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