Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Next Generation Sequencing of LdlrKO LXRα-phosphorylation disrupted macrophage transcriptomes

ABSTRACT: Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. Besides ligand binding, LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess changes in bone marrow derived macrophage transcriptional profiles of mice that carry LysMcre directed phosphorylation deficient-version of LXRα compared (S196A) to wild-type (WT).

ORGANISM(S): Mus musculus

PROVIDER: GSE112213 | GEO | 2018/06/29

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Next Generation Sequencing of Wild Type and LXRα-Ser196 phosphorylation deficient Murine Hepatic Transcriptomes on a Chow diet

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess changes in the hepatic transcriptional profiles of mice that carry a whole-body phosphorylation deficient knock in mutant of LXRα (S196A) compared to wild-type (WT) fed a chow diet.

2019-01-21 | GSE96650 | GEO

Next Generation Sequencing of Wild Type and LXRα-Ser196 phosphorylation deficient Murine Hepatic Transcriptomes on a High Fat/High Cholesterol Diet

2019-01-21 | GSE95359 | GEO

Impaired Liver X Receptor alpha phosphorylation affects hepatic chromatin H3K27 acetylation patterns

Project description:Liver X Receptors (LXRα and β) are ligand-activated transcription factors that play a key role in the control of lipid homeostasis, as well as modulation of immunity and inflammation. Besides ligand binding, LXR activity can be regulated by posttranslational modifications, such as phosphorylation. This study aims to assess how changes in the phosphorylation status of LXRα affect the H3K27Ac histone modification, a known marker for active regulatory elements in promoters and enhancers, in livers of mice mice that carry a whole-body phosphorylation deficient-version of LXRα (S196A) compared to wild-type (WT), in response to a high fat-high cholesterol diet.

2019-01-22 | GSE114104 | GEO

Next Generation Sequencing of LdlrKO LXRα-phosphorylation disrupted macrophage transcriptomes

Project description:Next Generation Sequencing of LdlrKO LXRα-phosphorylation disrupted macrophage transcriptomes

| PRJNA445274 | ENA

Foam cell specific LXRα ligand

Project description:OBJECTIVE: The liver X receptor α (LXRα) is a ligand-dependent nuclear receptor and the major regulator of reverse cholesterol transport in macrophages. This makes it an interesting target for mechanistic study and treatment of atherosclerosis. METHODS AND RESULTS: We optimized a promising stilbenoid structure (STX4) in order to reach nanomolar effective concentrations in LXRα reporter-gene assays. STX4 displayed the unique property to activate LXRα effectively but not its subtype LXRβ. The potential of STX4 to increase transcriptional activity as an LXRα ligand was tested with gene expression analyses in THP1-derived human macrophages and oxLDL-loaded human foam cells. Only in foam cells but not in macrophage cells STX4 treatment showed athero-protective effects with similar potency as the synthetic LXR ligand T0901317 (T09). Surprisingly, combinatorial treatment with STX4 and T09 resulted in an additive effect on reporter-gene activation and target gene expression. In physiological tests the cellular content of total and esterified cholesterol was significantly reduced by STX4 without the undesirable increase in triglyceride levels as observed for T09. CONCLUSIONS: STX4 is a new LXRα-ligand to study transcriptional regulation of anti-atherogenic processes in cell or ex vivo models, and provides a promising lead structure for pharmaceutical development.

2013-09-05 | GSE39079 | GEO

LXRα genomic binding in murine macrophages

Project description:Liver X receptors (LXRα and LXRβ) are nuclear receptor transcription factors that play crucial roles in regulating metabolic and immune functions in macrophages. Both receptors are present in macrophages and exhibit significant sequence similarity, yet their specific roles in various macrophage models, especially in inflammatory contexts, remain underexplored. Prior research has demonstrated that ligand-activated LXRs can suppress the expression of pro-inflammatory cytokines in macrophages. Here, we explored the genomic landscape of LXRa under inflammatory conditions using BMDM lacking LXRb as previously described (de la Rosa et al 2024); we treated BMDM with synthetic LXR ligand GW3965 alone or in combination with lipopolysaccharide for 24h.

2024-08-27 | GSE275506 | GEO

Transcriptome sequencing (RNA-Seq) of kidney and liver tissues from Lxrα-/- mices or wild type mices.

Project description:As a cholesterol sensor, nuclear receptor liver X receptor (Lxr) activated by oxysterols is an important factor regulating cholesterol homeostasis and plays central roles in the transcriptional control of cholesterol metabolism. Lxrα is one of the liver X receptor(Lxrs) subtypes. Lxrα is abundantly expressed in liver, small intestine, adipose, kidney, adrenal tissues, and macrophages. Lxrα's regulation of lipid metabolism is well known but its regulation of other downstream genes remains unclear.In order to investigate the regulating effect of Lxrα on other genes, we used the second-generation transcriptome sequencing(RNA-seq) to determine the expression changes of each gene in Lxrα-/- mices comparing with wild type mices.

2024-10-30 | GSE161880 | GEO

Pioneer factor Foxa2 enables ligand-dependent activation of type II nuclear receptors FXR and LXRa

Project description:Type II nuclear hormone receptors, such as FXR, LXR, and PPAR, which function in glucose and lipid metabolism and serve as drug targets for metabolic diseases, are permanently positioned in the nucleus regardless of the ligand status. Ligand activation of these receptors is thought to occur by co-repressor/co-activator exchange, followed by initiation of transcription. However, recent genome-wide location analysis showed that LXRα and PPARα binding in the liver is largely ligand-dependent. We hypothesized that pioneer factor Foxa2 evicts nucleosomes to enable ligand-dependent receptor binding. We show that chromatin accessibility, FXR binding and LXRα occupancy, and ligand-responsive activation of gene expression by FXR and LXRα require Foxa2. Unexpectedly, Foxa2 occupancy is drastically increased when either receptor, FXR or LXRα, is bound by an agonist. In addition, co-immunoprecipitation experiments demonstrate that Foxa2 interacts with either receptor in a ligand-dependent manner, suggesting that Foxa2 and the receptor bind DNA as an interdependent complex during ligand activation. Furthermore, PPARα binding is induced in Foxa2 mutants treated with FXR and LXR ligands, leading to activation of PPARα targets.

2021-07-12 | GSE149075 | GEO

TLR-dependent transcriptional reprogramming of LXRα cistrome in bone marrow-derived macrophages

Project description:The Liver X receptors (LXRα and LXRβ) are members of the nuclear receptor superfamily of transcription factors, and play key roles in the coordination of both metabolic and immune responses in macrophages. Both LXRs are expressed in macrophages and they share a high degree of sequence homology but their individual roles in different models of macrophage cultures, particularly in response to inflammation, have not been characterized. Previous studies have shown that ligand pre-treated LXRs repress the induction of pro-inflammatory cytokines in macrophages. Here, we show evidence that the expression of LXRα (but not LXRβ) is transcriptionally regulated at late phases of an LPS-induced inflammatory response in bone marrow-derived macrophages (BMDMs). Regulation of LXRα expression is controlled at the transcriptional levels by inflammatory signaling networks. Transcriptional profiling studies and ChIP-seq data identified a number of innate immune pathways whose expression is substantially controlled by LXRα in BMDMs. In summary, our data shows that LXRα plays a previously unrecognized role in innate immunity by transcriptional regulation of a battery of inflammatory genes through TLR and Type-I IFN-dependent signaling in macrophages.

2020-05-01 | GSE130586 | GEO

TLR-dependent transcriptional reprogramming of LXRα cistrome in bone marrow-derived macrophages

2022-12-31 | GSE200922 | GEO