Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [ChIP-seq]

ABSTRACT: We performed ChIP-seq for EZH2 and its associated histone mark, H3K27me3, in T98G Glioblastoma multiforme cells.

ORGANISM(S): Homo sapiens

PROVIDER: GSE112240 | GEO | 2019/01/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [RNA-seq]

Project description:We performed RNA-seq to identify differentially expressed genes in response to knockout of EZH2 and global miRNA knockdown (VP55 overexpressed) in T98G Glioblastoma multiforme cells.

2019-01-09 | GSE112241 | GEO

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [iCLIP]

Project description:We performed iCLIP for AGO2 to identify AGO2-interacting mRNAs in T98G glioblastoma multiforme cells.

2019-01-09 | GSE112651 | GEO

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network.

Project description:Gene expression can be regulated at multiple levels, but it is not known if and how there is broad coordination between regulation at the transcriptional and post-transcriptional levels. Transcription factors and chromatin regulate gene expression transcriptionally, whereas microRNAs (miRNAs) are small regulatory RNAs that function post-transcriptionally. Systematically identifying the post-transcriptional targets of miRNAs and the mechanism of transcriptional regulation of the same targets can shed light on regulatory networks connecting transcriptional and post-transcriptional control. We used individual-nucleotide resolution UV crosslinking and immunoprecipitation (iCLIP) for the RNA-induced silencing complex (RISC) component AGO2 and global miRNA depletion to identify genes directly targeted by miRNAs. We found that Polycomb repressive complex 2 (PRC2) and its associated histone mark, H3K27me3, is enriched at hundreds of miRNA-repressed genes. We show that these genes are directly repressed by PRC2 and constitute a significant proportion of direct PRC2 targets. For just over half of the genes corepressed by PRC2 and miRNAs, PRC2 promotes their miRNA-mediated repression by increasing expression of the miRNAs that are likely to target them. miRNAs also repress the remainder of the PRC2 target genes, but independently of PRC2. Thus, miRNAs post-transcriptionally reinforce silencing of PRC2-repressed genes that are inefficiently repressed at the level of chromatin, by either forming a feed-forward regulatory network with PRC2 or repressing them independently of PRC2.

| S-EPMC6360819 | biostudies-literature

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-01-09 | GSE112242 | GEO

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [miRNA-seq]

Project description:We performed miRNA-seq to test for global loss of miRNA in response to VP55 overexpression.

2019-01-09 | GSE112612 | GEO

Bivalent chromatin domains in glioblastoma multiforme reveal an epigenetic signature of early neural development mediated by SHH and Wnt signaling

Project description:We performed ChIP-seq for H3K27me3 and H3K4me3 to identify bivalent chromatin regions in T98G Glioblastoma multiforme cells.

2018-04-19 | GSE113361 | GEO

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network

Project description:MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network

| PRJNA445335 | ENA

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [iCLIP]

Project description:MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [iCLIP]

| PRJNA448605 | ENA

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [RNA-seq]

Project description:MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [RNA-seq]

| PRJNA445338 | ENA

MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [ChIP-seq]

Project description:MicroRNAs reinforce repression of PRC2 transcriptional targets independently and through a feed-forward regulatory network with PRC2 [ChIP-seq]

| PRJNA445330 | ENA

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data