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Genome-wide mapping and functional analysis of DNA methylation in clonal invasive crayfish

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Procambarus virginalis Procambarus fallax

PROVIDER: GSE112411 | GEO | 2018/10/11

REPOSITORIES: GEO

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Genome-wide mapping and functional analysis of DNA methylation in clonal invasive crayfish [WGBS]

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Project description:DNA methylation is an important epigenetic modification that has been repeatedly implied in organismal adaptation. However, many previous studies that have linked DNA methylation patterns to environmental parameters have been limited by confounding factors, such as cell-type heterogeneity and genetic variation. In this study, we analyzed DNA methylation variation in marbled crayfish, a clonal and invasive freshwater crayfish that is characterized by a largely tissue-invariant methylome and negligible genetic variation. Using a capture-based subgenome bisulfite sequencing approach that covers a small, variably methylated portion of the marbled crayfish genome, we identified specific and highly localized DNA methylation signatures for specimens from geographically and ecologically distinct wild populations. These results were replicated both biologically and technically by re-sampling at different time points and by using independent methodology. Finally, we show specific methylation signatures for laboratory animals and for laboratory animals that were reared at a lower temperature. Our results thus demonstrate the existence of context-dependent DNA methylation signatures in a clonal animal.

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Genome-wide mapping and functional analysis of DNA methylation in clonal invasive crayfish

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