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Pancreatic islet expression profiling in diabetes prone C57BLKS/J mice vs C57BL6/J mice.

ABSTRACT: C57BLKS/J mice are susceptible to diabetes, because of islet dysfunction, whereas C57BL6/J mice are not. Differences in gene expression between the two strains may account for this sensitivity. Furthermore these differences may only be evident in the hyperstimulated (diabetic or hyperglycemic) state. To this end profiling islets from these two strains cultured in both low and high glucose may reveal the underlying mechanism. Keywords: Mouse strain comparison under different culture conditions

ORGANISM(S): Mus musculus

PROVIDER: GSE11257 | GEO | 2008/12/31

SECONDARY ACCESSION(S): PRJNA106769

REPOSITORIES: GEO

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Pancreatic islet expression profiling in diabetes prone C57BLKS/J mice vs C57BL6/J mice.

Project description:C57BLKS/J mice are susceptible to diabetes, because of islet dysfunction, whereas C57BL6/J mice are not. Differences in gene expression between the two strains may account for this sensitivity. Furthermore these differences may only be evident in the hyperstimulated (diabetic or hyperglycemic) state. To this end profiling islets from these two strains cultured in both low and high glucose may reveal the underlying mechanism. Keywords: Mouse strain comparison under different culture conditions In the study presented here, pancreatic islets from 20 mice grown in low and high glucose conditions were assayed for differences in gene expression. (five C57BLKS/J low glucose, four C57BLKS/J high glucose, six C57BL6/J low glucose, five C57BL6/J high glucose). Technical replicates are achieved by labeling each sample with both Cy3 and Cy5, and combining the values for each hybridization.

2008-12-30 | E-GEOD-11257 | biostudies-arrayexpress

Expression data from the transplanted mouse islets into hyperglycemic and normoglycemic mice at 6 hours post-transplantation

Project description:insulin treatment protects islets from the initial rapid loss that is usually observed after transplantation and positively affects the outcome of islet transplantation in Akita mice. To study the molecular mechanism underlying the improved islet survival, expression profile analyses was performed to analyze the differences between the grafts transplanted into hyperglycemic and normoglycemic mice at 6 hours post-transplantation Cells were harvested at grafts for RNA extraction and hybridization on Affymetrix microarrays. We performed gene expression profiles of the transplanted mouse islets into hyperglycemic and normoglycemic mice at 6 hours post-transplantation

2014-04-18 | E-GEOD-55764 | biostudies-arrayexpress

Expression data from the transplanted mouse islets into hyperglycemic and normoglycemic mice at 6 hours post-transplantation

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SM009 - SARS infection of C57BL6, and PLAT knock-out mice.

Project description:This purpose of this experiment was to investigate the transcriptional differences between C57BL6, PLAT knock-out mice infected with SARS MA15 virus.

2014-02-20 | GSE51387 | GEO

IM015 - Influenza infection of C57BL6 and RIPK3 knock-out mice

2014-04-02 | GSE51526 | GEO

SM004 - SARS infection of C57BL6, TIMP1 and Serpine1 knock-out mice.

Project description:This purpose of this experiment was to investigate the transcriptional differences between C57BL6, TIMP1 and Serpine1 knock-out mice infected with SARS MA15 virus.

2014-02-20 | GSE51386 | GEO

Mus musculus

Project description:Pancreatic islet expression profiling in diabetes prone C57BLKS/J mice vs C57BL6/J mice.

| PRJNA106769 | ENA

Project description:From the past reports, we hypothesized that spleen is an ideal site for inducing regeneration of transplanted islets, and leading to reduce the required number of islets for ameliorating the hyperglycemia of diabetic recipients in mice. In order to confirm this hypothesis, we performed 25 islets transplantation into spleen (SP25); it was not enough number to ameliorate the hyperglycemia of recipient mice, with 100 islets transplantation into beneath the kidney capsule (KC100) to maintain recipients' blood glucose normoglycemic temporary. All recipient mice (n=11) became normoglycemic after receiving SP25 with KC100. On 240 days after transplantation, we performed nephrectomy for removing islet grafts in the kidney. After nephrectomy, 8 of 11 mice remained normoglycemic, and 3 of 11 mice' non-fasting blood glucose levels were maintained around 300 mg/dL. On 290 days after transplantation (50 days after nephrectomy), all recipient mice received splenectomy to remove islet grafts in the spleen. All mice became hyperglycemic after splenectomy, indicating that intra-splenic islet grafts maintained the blood glucose levels of diabetic recipient mice. In order to investigate the gene expression associated with islets engraftment in the spleen, microarray studies were performed in comparison of the Tlx-1 (Hox11) related gene expression profiles of Sample 1, Sample 2 and Sample 3.

2017-03-01 | GSE84512 | GEO

SM009 - SARS infection of C57BL6, and PLAT knock-out mice.

Project description:This purpose of this experiment was to investigate the transcriptional differences between C57BL6, PLAT knock-out mice infected with SARS MA15 virus. Overview of Experiment: Groups of 20 week old C57BL6 and PLAT knock-out mice were infected with SARS MA15 virus. Infections were done at 10^5 PFU or time-matched mock infected. Time points were 4 and 7 d.p.i. There were 2-4 animals/dose/time point. Lung samples were collected for virus load, transcriptional and proteomics analysis. Weight loss and animal survival were also monitored.

2014-02-20 | E-GEOD-51387 | biostudies-arrayexpress

IM015 - Influenza infection of C57BL6 and RIPK3 knock-out mice

Project description:This purpose of this experiment was to investigate the transcriptional differences between C57BL6, RIPK3 knock-out mice infected with influenza strain A/CA/04/2009 (H1N1) virus. Overview of Experiment: Groups of 6-8 week-old C57BL6 and RIPK3 knock-out mice were infected with influenza A/CA/04/2009 virus. Infections were done at 10^5 PFU or time-matched mock infected. Time points were 2 and 4 d.p.i. There were 2-3 animals/dose/time point. Lung samples were collected for virus load and transcriptional analysis. Weight loss and animal survival were also monitored.

2014-04-02 | E-GEOD-51526 | biostudies-arrayexpress

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