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Genome wide mapping of R-loops in the repriming deficient human cells using RNA DIP-Seq

ABSTRACT: The goal of this study was to compare the qualitative and quantitative differences in the presence of DNA:RNA hybrids (R-loops) between human wild type and PrimPol knockout human iPS cell lines. Our initial genetic experiments on a reporter locus have showed a link between the formation of R-loop and non-B DNA motifs; we have therefore analysed the correlation between R-loop abundance and of sequences computationaly predicted to form triplex-DNA or G-quadruplex forming sequences, and whether this changes between the two cell lines.

ORGANISM(S): Homo sapiens Gallus gallus

PROVIDER: GSE112745 | GEO | 2018/11/26

REPOSITORIES: GEO

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