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Small RNA (sRNA) sequencing data from pre-meiotic and meiotic anthers of Asparagus

ABSTRACT: Purpose: To study pre-meiotic (21-nt) and meiotic (24-nt) phasiRNA pathways in non-grass monocots Methods: Anthers were dissected using a 2 mm stage micrometer in a stereo microscope, and immediately frozen in liquid nitrogen until total RNA isolation was performed. Small RNA, mRNA libraries were generated using short-read (Illumina) and Single Molecule Real Time SMRT (PacBio) sequencing approaches. Stages were assigned based on the morphology of archesporial (AR) and tapetal cells of A. officinalis (Asparagus) anthers.

ORGANISM(S): Asparagus officinalis

PROVIDER: GSE112800 | GEO | 2018/07/07

REPOSITORIES: GEO

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