Transcriptomics

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Transcriptome profiling of feeding-to-fasting transition in chicken liver using a chicken 20K oligo microarray


ABSTRACT: Four weeks old male broilers, fed ad libitum or fasted for 16h or 48h were used to describe the evolution of global gene expression profiles in chicken liver during a 48h fasting period using a chicken 20K oligo array. Among the 20460 oligos on the microarray, 13057 were identified as aligning with a unique coding region of the 2.1 Washington University assembly of the chicken sequence genome. So the statistical analyses were performed on this 13057 gene set. A total number of 2062 differentially expressed genes were identified. The number of genes differentially expressed after 48h of fasting compared to the Fed state was 4-fold higher than after 16h of fasting. Analysis was focused on 1162 genes selected among these 2062 genes for which a human ortholog could be identified, thus allowing functional information collect. Quantitative real-time polymerase chain reaction (qRT-PCR) validated our results. Keywords: Chicken fasted, transcriptional profiling, differentially expressed genes

ORGANISM(S): Gallus gallus

PROVIDER: GSE11290 | GEO | 2008/05/06

SECONDARY ACCESSION(S): PRJNA106705

REPOSITORIES: GEO

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