Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Identification of a miRNA Regulatory Network in FACS-Purified Neuronal Progenitors at the Onset of Rat Neurogenesis

ABSTRACT: Cortical development is a complex process involving the generation of neuronal progenitors, which proliferate and migrate to form the stratified layers of the maturing cortex. To identify microRNAs (miRNAs) and genes that may be important during early cortical development, we analyzed the expression profiles of rat neuronal progenitors obtained at embryonic day 11 (E11), E12 and E13 using microarrays. Neuronal progenitors were purified from telencephalic dissociates with a positive-selection strategy using surface labeling tetanus-toxin and cholera-toxin and fluorescence-activated cell sorting. We identified classes of miRNAs and mRNAs that were up-regulated or down-regulated in these neuronal progenitors as cortical development progressed from E11 to E13. We present data that supports a regulatory role for miRNAs during the transition from neuronal progenitors into differentiating cortical neurons. Keywords: Developmental

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE11334 | GEO | 2008/12/01

SECONDARY ACCESSION(S): PRJNA106661

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcription profiling of rat neuronal progenitors obtained at embryonic day 11 (E11), E12 and E13 identifies an miRNA Regulatory network at the onset of rat neurogenesis

Project description:Cortical development is a complex process involving the generation of neuronal progenitors, which proliferate and migrate to form the stratified layers of the maturing cortex. To identify microRNAs (miRNAs) and genes that may be important during early cortical development, we analyzed the expression profiles of rat neuronal progenitors obtained at embryonic day 11 (E11), E12 and E13 using microarrays. Neuronal progenitors were purified from telencephalic dissociates with a positive-selection strategy using surface labeling tetanus-toxin and cholera-toxin and fluorescence-activated cell sorting. We identified classes of miRNAs and mRNAs that were up-regulated or down-regulated in these neuronal progenitors as cortical development progressed from E11 to E13. We present data that supports a regulatory role for miRNAs during the transition from neuronal progenitors into differentiating cortical neurons. Experiment Overall Design: Flow cytometry was used to isolate tetanus toxin+ and cholera toxin+ neuronal progenitors from embryonic days 11, 12 and 13 rat telencephalon. 4 biological replicates were obtained for each group using a pooled litters for each biological replicate on different prepararation days

2009-01-13 | E-GEOD-11334 | biostudies-arrayexpress

Molecular Signatures and Cellular Diversity During Mouse Habenula Development

Project description:single-cell RNA sequencing data (plate-based CelSeq2) of developing mouse habenula. DevHb series contains E11, E12, E13, E15, E18, P4, P7 and adult Brn3a-tLacZ mouse habenula and E18 contains C57BL6 mouse habenula.

2022-02-16 | GSE188712 | GEO

MicroRNA profiling of neural progenitor cells obtained from the brain of E11 and E13 stage rats

Project description:microRNA expression profiles of neural progenitors obtained from E11 and E13 telencephalon rats.

2008-12-01 | E-MEXP-1596 | biostudies-arrayexpress

Study of miRNAs function in basal progenitors during cortical neurogenesis

Project description:During cortical development neurons are generated sequentially from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activaed cell sorting (FACS) to isolate BPs from Tbr2GFP knockin reporter mice (Arnold SJ et al. Genesis, 2009) at early (embryonic day, E13) and late (embryonic day, E16) stages of cortical neurogenesis and determined miRNA expression profiles using mouse miRNA microarray (Agilent).Comparison of E13 and E16 microRNA expression profiles allowed us to identify regulatory mechanisms for maintaining stage specific homeostasis of BPs. FACS isolated BPs at E13 and E16 mouse brain cortex were used for miRNA microarray analyses. Four biological replicates (embryonic cortex from three different litters) for each group (E13 or E16) were analysed.

2014-05-01 | E-GEOD-45449 | biostudies-arrayexpress

RNA-seq of mice genital ridges from males and females at embryonic days E11 and E12

Project description:The aim of the study was to identify differentially expressed genes, isoforms and AS modifications accompanying Gonadal Sex Determination in mice. We performed a Rna-Seq analysis of XX and XY gonads during sex determination on embryonic days 11 (E11) and 12 (E12). RNA-seq libraries were prepared from grouped gonads from E11 and E12 males and females. The cDNA libraries were sequenced using a HiSeq2500 v4 chemistry system

2019-02-21 | E-MTAB-7656 | biostudies-arrayexpress

Study of gene networks in basal progenitors during cortical neurogenesis

Project description:During cortical development neurons are generated sequentially from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activaed cell sorting (FACS) to isolate BPs from Tbr2GFP knockin reporter mice (Arnold SJ et al. Genesis, 2009) at early (embryonic day, E13) and late (embryonic day, E16) stages of cortical neurogenesis and determined mRNA expression profiles using mouse mRNA microarray (Illumina MouseWG-6 v2). Comparison of E13 and E16 mRNA expression profiles allowed us to identify regulatory gene networks for maintaining stage specific homeostasis of BPs throughout neurogenesis. FACS isolated BPs at E13 and E16 mouse brain cortex were used for microarray analyses. Six biological replicates (embryonic cortex from three different litters) for E13 and five biological replicates (embryonic cortex from three different litters) for E16 were analysed.

2014-05-01 | E-GEOD-45450 | biostudies-arrayexpress

Gene expression in basal progenitors in the cerebral cortex of Tbr2 conditional knockout mice

Project description:During cortical development neurons are generated from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activated cell sorting (FACS) to isolate BPs from Tbr2-conditional knockout mice brain at early (E13) and late (E16) stages of cortical neurogenesis and determined mRNA expression profiles using microarray (Illumina MouseWG-6 v2). Role of transcription factor Tbr2 in basal progenitors during corticogenesis.

2019-10-12 | GSE121180 | GEO

Study of miRNAs function in basal progenitors during cortical neurogenesis

2014-05-01 | GSE45449 | GEO

Study of gene networks in basal progenitors during cortical neurogenesis

Project description:During cortical development neurons are generated sequentially from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activaed cell sorting (FACS) to isolate BPs from Tbr2GFP knockin reporter mice (Arnold SJ et al. Genesis, 2009) at early (embryonic day, E13) and late (embryonic day, E16) stages of cortical neurogenesis and determined mRNA expression profiles using mouse mRNA microarray (Illumina MouseWG-6 v2). Comparison of E13 and E16 mRNA expression profiles allowed us to identify regulatory gene networks for maintaining stage specific homeostasis of BPs throughout neurogenesis.

2014-05-01 | GSE45450 | GEO

Transcriptome profiling of Clostridium tetani

Project description:Clostridium tetani produces the tetanus-causing tetanus toxin (TeNT), one of the most powerful bacterial toxins known to humankind. The regulation of toxin expression is complex and involves the alternative sigma factor TetR as well as other regulators. Here, we identified a novel regulatory molecule, a non-coding small regulatory RNA (sRNA), located in the 3’ untranslated region of the tent gene. We show with an antisense RNA approach and recombinant expression of the tent locus with and without the sRNA in C. tetani strains that the sRNA acts as a negative regulator of TeNT expression and modulates the growth pattern of C. tetani. Its possible role is to limit tetanus toxin levels in the exponential growth phase; thus, it might interlock bacterial growth and toxin production.

2020-07-31 | GSE150141 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data