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Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect [sRNA-Seq]

ABSTRACT: research was focus on the virus-derived sRNAs expression pattern in rice host or planthopper vector and host/vector gene expression pattern when infected with RSV

ORGANISM(S): Oryza sativa Nilaparvata lugens

PROVIDER: GSE113554 | GEO | 2018/12/26

REPOSITORIES: GEO

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Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect [RNA-Seq]

Project description:research was focus on the virus-derived sRNAs expression pattern in rice host or planthopper vector and host/vector gene expression pattern when infected with RSV

2018-12-26 | GSE113553 | GEO

Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect [sRNA-Seq]

Project description:Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect [sRNA-Seq]

| PRJNA451503 | ENA

Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect

Project description:Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect

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Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-12-26 | GSE113555 | GEO

Transcriptome analysis of rice stripe virus-infected rice

Project description:Rice stripe virus (RSV) is one of the major virus diseases of rice in East Asia. Rice plants infected with RSV usually show symptoms such as chlorotic leaf stripes, weakness and senescence of leaves, and dwarfism. In order to characterize the host response to RSV infection at the gene expression level, the changes in transcriptome profiles of RSV-infected rice were monitored at three, six, nine, twelve, and fifteen days after inoculation by a rice oligomicroarray. The microarray data indicated that 1. transcription, translation and protein processing machineries were activated, 2. chloroplasts were disintegrated, and mitochondrion function was activated, 3. genes for transporters and cell wall synthesis were suppressed, and 4. the expression levels of pathogenesis-related genes were changed by RSV infection. Concurrent observation of symptom development, virus accumulation and transcriptome profiles in RSV-infected plants indicates that RSV symptoms are caused by unbalanced activities of organelles, suppression of cell elongation, and uncontrolled water transport, while translation activity of host cells may be increased in correlation with RSV propagation. Keywords: time course, virus infection, disease response Comparison between RSV- and mock-infected rice. Biological replicates: 3 control, 3 infected, independently grown and harvested; 4 time points (3, 6, 9, 12 days after inoculation (DAI)). Biological replicate: 1 control, 1 infected, independently grown and harvested; 1 time point (15 DAI). 1 sample derived from 5 plants grown under the same conditons.

2009-09-01 | E-GEOD-12681 | biostudies-arrayexpress

Rice stripe virus-derived siRNAs play different regulatory roles in rice and in the insect vector Laodelphax striatellus.

Project description:BACKGROUND:Most plant viruses depend on vector insects for transmission. Upon viral infection, virus-derived small interfering RNAs (vsiRNAs) can target both viral and host transcripts. Rice stripe virus (RSV) is a persistent-propagative virus transmitted by the small brown planthopper (Laodelphax striatellus, Fallen) and can cause a severe disease on rice. RESULTS:To investigate how vsiRNAs regulate gene expressions in the host plant and the insect vector, we analyzed the expression profiles of small RNAs (sRNAs) and mRNAs in RSV-infected rice and RSV-infected planthopper. We obtained 88,247 vsiRNAs in rice that were predominantly derived from the terminal regions of the RSV RNA segments, and 351,655 vsiRNAs in planthopper that displayed relatively even distributions on RSV RNA segments. 38,112 and 80,698 unique vsiRNAs were found only in rice and planthopper, respectively, while 14,006 unique vsiRNAs were found in both of them. Compared to mock-inoculated rice, 273 genes were significantly down-regulated genes (DRGs) in RSV-infected rice, among which 192 (70.3%) were potential targets of vsiRNAs based on sequence complementarity. Gene ontology (GO) analysis revealed that these 192 DRGs were enriched in genes involved in kinase activity, carbohydrate binding and protein binding. Similarly, 265 DRGs were identified in RSV-infected planthoppers, among which 126 (47.5%) were potential targets of vsiRNAs. These planthopper target genes were enriched in genes that are involved in structural constituent of cuticle, serine-type endopeptidase activity, and oxidoreductase activity. CONCLUSIONS:Taken together, our results reveal that infection by the same virus can generate distinct vsiRNAs in different hosts to potentially regulate different biological processes, thus reflecting distinct virus-host interactions.

| S-EPMC6172784 | biostudies-literature

Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect [RNA-Seq]

Project description:Rice stripe virus-derived siRNAs play different regulatory roles in host plant and vector insect [RNA-Seq]

| PRJNA451497 | ENA

Transcriptome analysis of rice stripe virus-infected rice

2009-09-01 | GSE12681 | GEO

Integrative analysis of microRNAome and transcriptome elucidates the response of susceptible rice plants to rice stripe virus RSV (miRNA-Seq)

Project description:To investigate how rice responds to RSV infection, we integrated miRNA expression with parallel mRNA transcription profiling by deep sequencing. A total of 570 miRNAs were identified of which 69 miRNAs (56 up-regulated and 13 down-regulated) were significantly modified by RSV infection. Digital gene expression (DGE) analysis showed that 1274 mRNAs (431 up-regulated and 843 down-regulated genes) were differentially expressed by RSV infection. The differential expression of selected miRNAs and mRNAs was confirmed by qRT-PCR. Gene ontology (GO) and pathway enrichment analysis showed that a complex set of miRNA and mRNA networks were selectively regulated by RSV infection. In particular, 63 differentially expressed miRNAs were found to be significantly and negatively correlated with 160 target mRNAs. Interestingly, 22 up-regulated miRNAs were negatively correlated with 24 down-regulated mRNAs encoding disease resistance-related proteins, indicating that the host defense responses were selectively suppressed by RSV infection. The suppression of both osa-miR1423-5p- and osa-miR1870-5p-mediated resistance pathways was further confirmed by qRT-PCR. Chloroplast functions were also targeted by RSV, especially the zeaxanthin cycle, which would affect the stability of thylakoid membranes and the biosynthesis of ABA. All these modifications may contribute to viral symptom development and provide new insights into the pathogenicity mechanisms of RSV.

2017-09-01 | GSE74498 | GEO

Integrative analysis of microRNAome and transcriptome elucidates the response of susceptible rice plants to rice stripe virus RSV (RNA-Seq)

2017-09-01 | GSE74497 | GEO