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Effects of culture conditions on epigenomic profiles of brain tumor cells

ABSTRACT: We performed epigenomic analysis of brain tumor cells that were collected from micro-engineered three-dimensional tumor models. We used a low-input epigenomic analysis method known as microfluidic-oscillatory-washing-based chromatin immunoprecipitation with sequencing (MOWChIP-seq) to analyze genome-wide histone modification (H3K4me3). We compared H3K4me3 patterns in standard 2D cultures and 3D cultures based on type I collagen hydrogels, under both normoxic and hypoxic conditions. Our work illustrates a direct connection between cell culture or tissue niche condition and genome-wide alterations in histone modification.

ORGANISM(S): Homo sapiens

PROVIDER: GSE113824 | GEO | 2019/02/01

REPOSITORIES: GEO

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