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RNA-Sequencing analysis of differential gene transcription profiles induced by the prenatal/maternal broccoli sprout (BSp) diet

ABSTRACT: Purpose: We tested global gene transcriptome changes by RNA-sequencing analysis in the offspring breast tumors of SV40 transgenic mice to further identify key epigenetic-controlled genes in regulation of the prenatal/maternal BSp diet-mediated early breast cancer prevention. Method: Mouse offspring mammary tumor mRNA from control and maternal BSp treatment were generated by deep sequencing, in duplicate or triplicate, using Illumina NextSeq500 platform (GPL19057). The sequence reads that passed quality filters were analyzed. We utilized the R/Bioconductor package DESeq to evaluate differential gene expression for sequence count data by the use of negative binomial distributio. qRT–PCR validation was performed using TaqMan and SYBR Green assays. Conclusions: Our data showed differential transcriptome distribution in the breast tumors of mouse offspring between the control and prenatal/maternal BSp treatment groups.

ORGANISM(S): Mus musculus

PROVIDER: GSE113900 | GEO | 2018/05/02

REPOSITORIES: GEO

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RNA-Sequencing analysis of differential gene transcription profiles induced by the prenatal/maternal broccoli sprout (BSp) diet

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DNA methylation signatures triggered by prenatal maternal stress exposure to a natural disaster: Project Ice Storm

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