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Targeted sequencing based maternal whole blood expression changes with gestational age and labor in normal pregnancy

ABSTRACT: Maternal plasama colected longitudinally were profiled using targeted sequencing (DriverMap™) (https://www.cellecta.com) to evaluate changes with gestational age and with labor in normal pregnancy.

ORGANISM(S): Homo sapiens

PROVIDER: GSE114037 | GEO | 2019/02/04

REPOSITORIES: GEO

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Maternal whole blood expression changes with gestational age and labor in normal pregnancy [array]

Project description:Maternal plasama colected longitudinally were profiled using Affymetrix Human Transcriptome Arrays to evaluate changes with gestational age and with labor in normal pregnancy.

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Maternal whole blood transcriptomics to assess changes with gestational age in normal pregnancy I

Project description:Maternal whole blood colected longitudinally were profiled using Affymetrix Human Transcriptome Arrays to evaluate changes with gestational age and with labor in normal pregnancy. This is a re-analysis of GSE113809, with additional metadata and different summarization of gene expression at ENTREZ ID level instead of Transcript Cluster as in the original submission. LMP is the date at the last menstrual period. Oper_LMP is the date at the last menstrual period with eventual adjustment based on ultrasound determination. Gestational age (GA) (weeks) is defined as (Date at sample - Oper_LMP)/7. For control pregnancies, SubGroup defines those with spontaneous labor at term (TL) as opposed with those delivered by cesarean section (TNL). Train is 1 if the sample was used in sub-challenge 1 in the training set as opposed to test set (0). D2 is 1 if the sample was used in the 2 interval prediction scenario, and if yes, it was assigned to the interval given by column D2Interval. D3 is 1 if the sample was used in the 3 interval prediction scenario, and if yes, it was assigned to the interval given by column D3Interval. DiagnosisGA is the gestational age at diagnosis with any disease indicated by the Group variable, and it is set to NA for normal pregnancies.

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Maternal whole blood transcriptomics to assess changes with gestational age in normal pregnancy II

Project description:Maternal whole blood colected longitudinally were profiled using Affymetrix Human Transcriptome Arrays . This is a re-analysis of GSE121974, with additional metadata and different summarization of gene expression at ENTREZ ID level instead of Transcript Cluster as in the original submission. LMP is the date at the last menstrual period. Oper_LMP is the date at the last menstrual period with eventual adjustment based on ultrasound determination. Gestational age (GA) (weeks) is defined as (Date at sample - Oper_LMP)/7. For control pregnancies, SubGroup defines those with spontaneous labor at term (TL) as opposed with those delivered by cesarean section (TNL). Train is 1 if the sample was used in sub-challenge 1 in the training set as opposed to test set (0). D2 is 1 if the sample was used in the 2 interval prediction scenario, and if yes, it was assigned to the interval given by column D2Interval. D3 is 1 if the sample was used in the 3 interval prediction scenario, and if yes, it was assigned to the interval given by column D3Interval. DiagnosisGA is the gestational age at diagnosis with any disease indicated by the Group variable, and it is set to NA for normal pregnancies.

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Maternal whole blood expression changes with gestational age and labor in normal pregnancy

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Targeted sequencing based maternal whole blood expression changes with gestational age and labor in normal pregnancy

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Transcriptomic phenotyping of human labor myometrium.

Project description:Myometrial biopsies were collected from 31 women undergoing primary cesarean sections and were carefully phenotyped with respect to gestational age (GA), circumstances of labor onset, and clinical status at the start and end of the intervention. Cases were aggregated into groups as follows: Group 1: term birth following spontaneous onset of term labor (TL, n=5); Group 2: term birth by elective cesarean section not in labor (TNL, n=5); Group 3: PTB following spontaneous preterm labor with intact membranes (n=6); Group 4: preterm birth following PPROM (n=8); and Group 5: provider-initiated preterm birth in the absence of active labor contractions, cervical dilation or membrane rupture (n=7). Additional phenotyping of cases spontaneously committed to PTBs (Groups 3 and 4) involved presence or absence of Triple I based on cultures of amniotic fluid obtained via clinically-indicated amniocentesis. RNAs were profiled using second generation RNA sequencing.

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