Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Circular RNA expression alteration in rats after traumatic spinal cord injury (SCI)

ABSTRACT: To determine whether the expression levels of circular RNAs were altered and lay a foundation for future work, we used high-throughput microarray analysis to screen circular RNAs expression patterns in the spinal cord of adult rats after traumatic spinal cord injury (SCI), finally to evaluate the potential rat models as a platform for the development of novel therapeutic targets for spinal cord injury in future clinical studies. Overall six rats at 3 days post-SCI in two groups were used to perform the microarray.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE114426 | GEO | 2018/05/15

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Circular RNA expression alteration in rats after traumatic spinal cord injury (SCI)

Project description:Circular RNA expression alteration in rats after traumatic spinal cord injury (SCI)

| PRJNA471324 | ENA

Circular RNAs expression profiles and potential key molecules incompletely transected spinal cord injury.

Project description:Circular RNA is implicated in numerous diseases and conditions, including traumatic injury to the central nervoussystem. However, we know little regarding their role in completely transected spinal cord injury (SCI). Our studyused high-throughput sequencing to analyze circular RNA expression in rats after experimental completetransection of spinal cord. We found differential expression in 400 circular RNAs after SCI, with 249 significantlyup-regulated and 151 significantly down-regulated. We then selected five circular RNAs for qRT-PCR validation,and the results were in agreement with RNA sequencing. Additionally, we predicted the function of circular RNAin SCI through GO and KEGG analyses, as well as the construction of circRNA/microRNA interaction networks.Furthermore, we identified circular RNA chr9:1829226-1834212 and mRNA Hdac11 as key molecules after SCI.In conclusion, this study is the first to characterize circular RNA function in rats after completely transected SCI,through identifying their differential expression. Our results provide insight into the mechanism and therapeutictargets of SCI.

2020-08-28 | GSE156999 | GEO

Differential Expression profiles of tRNA-Derived Small RNAs in Rats After Traumatic Spinal Cord Injury

Project description:The goals of this study are to screen differential expression profiles of tRNA-Derived small RNAs (tsRNAs) in rats after traumatic spinal cord injury (SCI) using next-generation sequencing and qRT-PCR.As a result, in rat spinal cord 1 day after contusion, totally 297 tsRNAs differentially expressed were identified. 155 tsRNAs were significantly differentially expressed: 91 were significantly up-regulated, while 64 were significantly down-regulated after SCI (folding change>1.5; P<0.05).Next, four tsRNAs were selected to be verified by qRT-PCR. Taken together,this study explored, for the first time, the significant alteration of tsRNA expression profiles after traumatic SCI in rats and offered deep insights into many possible treatment targets of SCI by regulating tsRNAs.

2020-01-24 | GSE133157 | GEO

Novel targets in injured cord in an obese SCI rat model

Project description:In the present study, we sought to understand the impact of obesity/metabolic disease (high-fat induced) on spinal cord injury (SCI) by examining transcriptome. Adult, male Long Evans rats received either thoracic level contusion of the spinal cord or sham laminectomy and then were allowed to recover on normal rat chow for 4 weeks and further on HFD for an additional 8 weeks. Spinal cord tissues harvested from the rats were processed for Affymetrix microarray and further transcriptomic analysis.

2018-01-01 | GSE102964 | GEO

Single nucleus RNAseq of mouse astrocytes after traumatic spinal cord injury

Project description:We conducted snRNAseq of mouse astrocytes after traumatic spinal cord injury (SCI). These data reveal transcriptomic similarities and differences among astrocytes in healthy spinal cord and after spinal cord injury.

2024-01-09 | GSE247844 | GEO

Transcriptional study of the temporal evolution after severe traumatic spinal cord injury (SCI) and neural progenitor cells (NPCs)-transplantation intervention.

Project description:The present study is intended to disclose the transcriptional profile of the temporal evolution through the different stages after SCI as well as the molecular processes underlying the NPC based-therapy in order to transcriptionally characterize its therapeutical mechanism. This transcriptional profile analysis of total RNA samples from spinal cord homogenates of adult rats (Sprague Dawley) provides tissular (tought not cell-type specific) information of the critical time points after the injury from 1 to 8 weeks (acute, sub-acute and early-chronic and late-chronic stages) which conferred a wide temporal coverage making it ideal for studying the temporal dynamics of SCI. Furthermore, we have evaluated the impact of intramedullary acute or subacute transplantation of NPCs over the transcriptional regulation of the spinal cord tissue in order to define the functional outcomes of the NPCs therapy.

2022-08-16 | GSE183591 | GEO

Identification of regulated genes associated with ketogenic diet treatment following SCI

Project description:To evaluate the changes in gene expression after spinal cord injury (SCI) and treatment with a Ketogenic Diet or a Control Diet (Refs., CD: F5960, Bioserv®; KD: F5848, Bioserv®), gene expression was studied 7 days after SCI surgery in rats. A total of 8 adult male Sprague-Dawley rats (~300 g at time of injury; Harlan Breeding Laboratory) were group-housed (21°C; 12 h: 12 h light:dark cycle). After a unilateral C5 laminectomy -dorsal processes of C4-C6 were held with a clamp and fixed in a frame tilted at a 22.5° angle and the cord was consutesd with a force of 150 Kdynes using the Infinite Horizon impactor- the animals were given ad libitum access to the 2 diets. Total RNA was isolated from 5-mm segment centered on the site of impact.

2021-08-11 | GSE159555 | GEO

Differential gene expression following SCI in trkB.T1 null

Project description:We asked what genes are significantly differentially regulated in the spinal cord of SCI trkB.T1 WT and trkB.T1 KO mice. TrkB.T1 is upregulated shortly after SCI although the precise mechanisms underyling this upregulation are poorly understood. In the trkB.T1 null, we show less mechanical allodynia and better locomotor recovery following SCI. The microarray studies helped us to elucidate a signaling pathway that is differently regulated in the WT versus KO mice at 1 day after SCI. In this study, we did not examine gene changes within a genotype after SCI. Rather, we examined DGE by genotype at each time point. Spinal cord tissue from WT and KO mice in a sham condition (intact spinal cord) versus 1D, 3D and 7D following SCI was harvested for microarray analyses.

2015-12-07 | E-GEOD-42828 | biostudies-arrayexpress

Screening and characterizing the effects of mmu_circ_0000296 on spinal cord injury

Project description:Spinal cord injury (SCI) is a common pathologic disorder that often presents with sensory and motor deficits, severely affecting patients' daily lives. Circular RNAs (circRNAs), a class of non-coding RNAs, have been reported to be involved in regulating the development of SCI. We used RNA sequencing technology and bioinformatics analysis to find the expression and regulation patterns of circRNAs and mRNAs in a mouse model of SCI, from which we screened the potential RNA-mmu_circ_0000296 that affects the progression of SCI in the acute phase. Based on STRING, ENCORI, miRTarBase database and Cytoscape software to construct the relational network of endogenous competing RNAs (ceRNAs) and protein-protein interaction (PPI) network. Finally, in order to validate the above bioinformatics results, the differential expression of circ_0000296 was verified in mouse SCI model and LPS-induced cell model, respectively. Not only that, in in vitro experiments, we found that circ_0000296 overexpression could inhibit microglia polarization toward M1 type, reduce inflammation level, and then inhibit neuronal apoptosis. These findings will help to elucidate and refine the effects of circRNAs on the pathophysiological progression of spinal cord injury and suggest that mmu_circ_0000296 may be one of the targets for spinal cord injury therapy.

2023-10-31 | GSE245437 | GEO

Brainstem control of transcription after spinal cord injury (SCI)

Project description:Brainstem control of transcription after spinal cord injury (SCI)

| PRJNA549934 | ENA