Global transcriptome analysis of resistant and susceptible soybean lines following Sclerotinia sclerotiorum infection
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ABSTRACT:
ORGANISM(S): Glycine max
PROVIDER: GSE114448 | GEO | 2018/11/19
REPOSITORIES: GEO
Similar Datasets
Project description:Sclerotinia sclerotiorum is a broad-host range necrotrophic pathogen which is the causative agent of Sclerotinia stem rot (SSR), and a major disease of soybean (Glycine max). A time course transcriptomic analysis was performed in both compatible and incompatible soybean lines to identify pathogenicity and developmental factors utilized by S. sclerotiorum to achieve pathogenic success.
2019-03-18 | GSE121983 | GEO
Project description:This SuperSeries is composed of the following subset Series: GSE15337: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 1 GSE15338: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 3 GSE15339: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 4 GSE15340: Gene expression profiling soybean stem tissue early response to Sclerotinia sclerotiorum 2 Refer to individual Series
2010-03-23 | E-GEOD-15369 | biostudies-arrayexpress
Project description:Quantitative disease resistance (QDR) is an almost universal and often broad-spectrum process in plants, serving to limit the damage caused by pathogen infections. It represents the primary form of plant immunity that reduces disease symptoms induced by numerous pathogens actively killing host cells during infection, including the necrotrophic pathogen Sclerotinia sclerotiorum. Investigating the evolutionary origins of QDR against necrotrophic fungi is crucial for comprehending how plant resistance evolves. To explore the diversity of local responses to S. sclerotiorum within a plant species level, we conducted a comprehensive analysis of the entire transcriptomes from 23 accessions of Arabidopsis thaliana, mainly distributed across Europe. More than half on the pan-transcriptome displayed local responses toS. sclerotiorum, including similar transcriptome patterns. Notably, core S. sclerotiorum-responsive genes exhibited a clear gene age pattern, dominated by older genes forming protein-protein networks that continuously acquiring new hubs. Comparative transcriptome analyses revealed QDR is associated with quantitative expression variations specific to accession subsets. By comparison of promoter sequences, we have shown evidence that accession subsets independently evolved and acquired specific cis-regulatory elements, confering Sclerotinia resistance. This scenario suggests multiple exaptation trajectories of novel QDR genes through species-level cis-regulation. This study sheds light on the regulation of QDR-associated genes within a species, contributing to our understanding of the molecular mechanisms of plant fungal resistance.
2024-02-27 | GSE248079 | GEO
Project description:MicroRNAs are multifunctional non-coding short nucleotide molecules. Nevertheless, the role of miRNAs in the interactions between plants and necrotrophic pathogens is largely unknown. Here, we report the identification of the miRNA repertoire of the economically important oil crop oilseed rape (Brassica napus) and those involved in interacting with its most devastating necrotrophic pathogen Sclerotinia sclerotiorum. We identified 280 B. napus miRNA candidates, including 53 novel candidates and 227 canonical members or variants of known miRNA families, by high-throughput deep sequencing of small RNAs from both normal and S. sclerotiorum-inoculated leaves. Target genes of 15 novel candidates and 222 known miRNAs were further identified by sequencing of degradomes from the two types of samples. MicroRNA microarray analysis revealed that 68 miRNAs were differentially expressed between S. sclerotiorum-inoculated and uninoculated leaves. A set of these miRNAs target genes involved in plant defense to S. sclerotiorum and/or other pathogens such as NBS-LRR R genes and nitric oxygen and reactive oxygen species related genes. Additionally, three miRNAs target AGO1 and AGO2, key components of post-transcriptional gene silencing (PTGS). Expression of several viral PTGS suppressors reduced resistance to S. sclerotiorum. Arabidopsis mutants of AGO1 and AGO2 exhibited reduced resistance while transgenic lines over-expressing AGO1 displayed increased resistance to S. sclerotiorum in an AGO1 expression level-dependent manner. Moreover, transient over-expression of miRNAs targeting AGO1 and AGO2 decreased resistance to S. sclerotiorum in oilseed rape. Our results demonstrate that the interactions between B. napus and S. sclerotiorum are tightly regulated at miRNA level and probably involve PTGS.
2017-05-16 | GSE81560 | GEO
Project description:MicroRNAs are multifunctional non-coding short nucleotide molecules. Nevertheless, the role of miRNAs in the interactions between plants and necrotrophic pathogens is largely unknown. Here, we report the identification of the miRNA repertoire of the economically important oil crop oilseed rape (Brassica napus) and those involved in interacting with its most devastating necrotrophic pathogen Sclerotinia sclerotiorum. We identified 280 B. napus miRNA candidates, including 53 novel candidates and 227 canonical members or variants of known miRNA families, by high-throughput deep sequencing of small RNAs from both normal and S. sclerotiorum-inoculated leaves. Target genes of 15 novel candidates and 222 known miRNAs were further identified by sequencing of degradomes from the two types of samples. MicroRNA microarray analysis revealed that 68 miRNAs were differentially expressed between S. sclerotiorum-inoculated and uninoculated leaves. A set of these miRNAs target genes involved in plant defense to S. sclerotiorum and/or other pathogens such as NBS-LRR R genes and nitric oxygen and reactive oxygen species related genes. Additionally, three miRNAs target AGO1 and AGO2, key components of post-transcriptional gene silencing (PTGS). Expression of several viral PTGS suppressors reduced resistance to S. sclerotiorum. Arabidopsis mutants of AGO1 and AGO2 exhibited reduced resistance while transgenic lines over-expressing AGO1 displayed increased resistance to S. sclerotiorum in an AGO1 expression level-dependent manner. Moreover, transient over-expression of miRNAs targeting AGO1 and AGO2 decreased resistance to S. sclerotiorum in oilseed rape. Our results demonstrate that the interactions between B. napus and S. sclerotiorum are tightly regulated at miRNA level and probably involve PTGS.
2017-05-16 | GSE81561 | GEO
Project description:Background: Sclerotinia sclerotiorum is one of the most severe fungus diseases in many important crops including sunflower (Helianthus annuus) worldwide. Breeding the resistant varieties are the best strategy to control S. sclerotiorum, however, the molecular regulatory mechanisms of sunflower in response to S. sclerotiorum remain poorly understood. Methods: We performed a leaf transcriptomic analysis to understand the differential defense response to S. sclerotiorum in a disease resistant (DR) genotype and a disease susceptible (DS) genotype of sunflower 24 h post-inoculation. Results: A total of 7439 and 10151 differentially expressed genes (DEGs) were identified in DR and DS genotypes, respectively suggesting that the former is less affected by S. sclerotiorum infection. Pathway analysis revealed that response to S. sclerotiorum was mostly enriched in the DEGs that are involved in the cell wall, redox homeostasis, immune response, protein kinase activity, hormone, transcription factor activities as well as secondary metabolism. The magnitude of expression changes in a set of genes encoding expansins, pectate lyase activities, antioxidant activity and ethylene following S. sclerotiorum infection likely contributed to the different resistance levels of these two genotypes. One gene encoding effector receptor NLR revealing significant opposite expression pattern between DR and DS genotypes along with three hub-genes CDPK9, MAPK5, CML23 by protein-protein interaction (PPI) network analysis were considered as the most possible candidate genes. Conclusion: Our results provide in-depth insights into the molecular mechanisms associated with sunflower defense responses against S. sclerotiorum that will be of great utility in Sclerotinia-resistance breeding.
2022-12-07 | GSE220161 | GEO
Project description:Sclerotinia sclerotiorum isolates resequencing
| PRJNA342788 | ENA
Project description:White mold disease, caused by Sclerotinia sclerotiorum (Lib) de Bary, can be a serious disease of crops grown under cool moist environments. In many plants, like soybean [Glycine max (L.) Merr.], complete genetic resistance does not exist. To identify possible genes involved in defense against this pathogen, and to determine possible physiological changes that occur during infection, a microarray screen was conducted using stem tissue to evaluate changes in gene expression between partially resistant and susceptible soybean genotypes at 8 and 14 hours post inoculation. RNA from 15 day old inoculated plants was labeled and hybridized to soybean cDNA microarrays. ANOVA identified 1270 significant genes from the comparison between time points and 105 genes from the comparison between genotypes. Selected genes were classified into functional categories. The analyses identified changes in cell-wall composition and signaling pathways, as well as suggesting a role for anthocyanin and anthocianidin synthesis in the defense against S. sclerotiorum. In-silico mapping of both the differentially expressed transcripts and of public markers associated with partial resistance to white mold, provided evidence of several differentially expressed genes being closely positioned to white mold resistance markers, with the two most promising genes encoding a PR-5 and anthocyanidin synthase. Keywords = white mould Keywords = necrotroph Keywords = oxalic acid Keywords = defense Keywords = ANOVA Keywords = PI 194639 Keywords = plant Keywords: plant microbe interaction loop design, 4 comparisons, 2 technical repeats including dye swaps, 3 biological repeats
2010-03-23 | E-GEOD-15339 | biostudies-arrayexpress
Project description:White mold disease, caused by Sclerotinia sclerotiorum (Lib) de Bary, can be a serious disease of crops grown under cool moist environments. In many plants, like soybean [Glycine max (L.) Merr.], complete genetic resistance does not exist. To identify possible genes involved in defense against this pathogen, and to determine possible physiological changes that occur during infection, a microarray screen was conducted using stem tissue to evaluate changes in gene expression between partially resistant and susceptible soybean genotypes at 8 and 14 hours post inoculation. RNA from 15 day old inoculated plants was labeled and hybridized to soybean cDNA microarrays. ANOVA identified 1270 significant genes from the comparison between time points and 105 genes from the comparison between genotypes. Selected genes were classified into functional categories. The analyses identified changes in cell-wall composition and signaling pathways, as well as suggesting a role for anthocyanin and anthocianidin synthesis in the defense against S. sclerotiorum. In-silico mapping of both the differentially expressed transcripts and of public markers associated with partial resistance to white mold, provided evidence of several differentially expressed genes being closely positioned to white mold resistance markers, with the two most promising genes encoding a PR-5 and anthocyanidin synthase. Keywords = white mould Keywords = necrotroph Keywords = oxalic acid Keywords = defense Keywords = ANOVA Keywords = PI 194639 Keywords = plant Keywords: plant microbe interaction 1 comparison, 2 technical repeats including dye swaps, 3 biological repeats
2010-05-05 | E-GEOD-15337 | biostudies-arrayexpress