Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Differential roles of TAp63α and deltaNp63α in chicken myogenic differentiation

ABSTRACT: We constructed chicken TAp63α and deltaNp63α overexpression vectors, and then transfected into chicken primary myoblast. After 48 hours transfection, we collected total RNA and conducted RNA-sequencing by using BGISEQ-500. GFP overexpression vector was used as control.

ORGANISM(S): Gallus gallus

PROVIDER: GSE114452 | GEO | 2018/05/16

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic analyses of co-infection of Mycoplasma gallisepticum and Escherichia coli in chicken lungs

Project description:Purpose:In order to assess the respiratory toxicity of co-infection in chicken lung, we established a co-infection model to investigate transcriptome profiles of chicken lung. Methods: RNA extracted by Trizol reagent (Invitrogen) was utilized to construct the final library (BGISEQ-500 RNA-Seq Library) based on the manufacturer’s instructions. Library was validated on the Agilent Technologies 2100 bioanalyzer. The library was amplified with phi29 to make DNA nanoball (DNB) which had more than 300 copies of one molecule. The DNBs were load into the patterned nanoarray and single end 50 bases reads were generated in the way of sequenced by synthesis. Whole transcriptome sequencing data was filtered and mapped to Chicken genome (Gallus genome Version 5.0.1 NCBI). DEG-seq method was based on Poisson distribution (Fold Change > 2 and Adjusted P value < 0.001) Results: Sampling directly from the lung yielded sufficient quantities of RNA to assess transcripts from each chicken and mapped to 18,043 Gallus gallus genes. Conclusions: We used the method of RNA-seq to find the target genes and related signaling pathways involved in the co-infection (MG and E.coli) and their underlying mechanisms.

2019-04-19 | GSE130015 | GEO

The Role of CD83 in ovarian cancer cell line SKOV3

Project description:We performed RNA-Seq of CD83 overexpression SKOV3 cells (OV_1, OV_2, OV_3), CD83 knockdown SKOV3 cells (KD_1, KD_2, KD-3), and control SKOV3 cells (NC_1, NC_2, NC_3) using BGISEQ-500 platform (BGI, China).

2019-01-14 | GSE125011 | GEO

BGISEQ-500 WGS demo FASTQ data

Project description:BGISEQ-500 WGS demo FASTQ data

| PRJEB15427 | ENA

Developmental dynamics of miRNAs across chicken pectoral muscle and effect of gga-miRNA-454 on the differentiation of myoblasts

Project description:Developmental dynamics of miRNAs across chicken pectoral muscle and effect of gga-miRNA-454 on the differentiation of myoblasts inhibits myoblast differentiation by targeting SBF2

2020-06-17 | GSE139304 | GEO

Differential roles of TAp63α and deltaNp63α in chicken myogenic differentiation

Project description:Differential roles of TAp63α and deltaNp63α in chicken myogenic differentiation

| PRJNA471417 | ENA

BGISEQ-500 ChIP-Seq SE50 demo data

Project description:BGISEQ-500 ChIP-Seq SE50 demo data

| PRJEB20179 | ENA

BGISEQ-500 RNA-seq demo FASTQ data

Project description:BGISEQ-500 RNA-seq demo FASTQ data

| PRJEB15426 | ENA

BGISEQ-500 RNA-Seq

Project description:The performance of BGISEQ-500 for RNA-Seq