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Rapid high-resolution measurement of DNA replication timing by droplet digital PCR

ABSTRACT: Time of DNA replication is inversely proportional to the relative DNA copy number. Sort-seq is a technique that uses deep short read sequencing to determine relative copy number across genome from sorted S phase cells. We have applied sort-seq to HeLa cells and report their relative copy number profile. The high (close to 2) value means the region replicates early in S phase; the low (close to 1) value means the region is replicated late in S phase.

ORGANISM(S): Homo sapiens

PROVIDER: GSE114480 | GEO | 2018/05/16

REPOSITORIES: GEO

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