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Genome wide analysis of chick limb bud tissue treated with FGF2 for 24hrs (microarray)

ABSTRACT: During limb development, fibroblast growth factors (FGFs) govern proximal-distal outgrowth and patterning. FGFs also synchronize developmental patterning between the proximal-distal and anterior-posterior axes by maintaining sonic hedgehog (SHH) expression in cells of the zone of polarizing activity (ZPA) in the distal posterior mesoderm. SHH, in turn, maintains FGFs in the apical ectodermal ridge (AER) which caps the distal tip of the limb bud. Crosstalk between FGF and SHH signaling is critical for patterned limb development, but the mechanisms underlying this feedback loop are not well characterized. Implantation of FGF beads in the proximal posterior limb bud can maintain SHH expression in the former ZPA domain (evident 3hrs after application), while prolonged exposure (24hrs) can induce SHH outside of this domain. Although temporally and spatially disparate, comparative analysis of transcriptome data from these different populations enriched molecules required for FGF-mediated SHH regulation.

ORGANISM(S): Gallus gallus

PROVIDER: GSE114661 | GEO | 2018/12/18

REPOSITORIES: GEO

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Meis genes regulate RA/FGF signaling to control multiple steps of limb development

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Integration of Shh and Fgf signaling in controlling Hox expression in cultured limb cells [ChIP-seq]

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