Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Gene expression in human lung tissue infected with mycobacterium tuberculosis

ABSTRACT: Differential gene expression in human lung tissue 24 hrs after infection compared to uninfected tissue from the same donor

ORGANISM(S): Homo sapiens

PROVIDER: GSE114911 | GEO | 2018/05/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

TaqMan Human miRNA array profile of Mycobacterium tuberculosis H37Rv infected THP-1 differentiated macrophages cells.

Project description:Differentially regulated miRNA candidates in H37Rv infected THP-1 cells were analysed with respect to uninfected THP-1 reference samples. THP-1 cells are monocytes differentiated to macrophages after treatment with PMA for 48 hrs. Total RNA was isolated from infected THP-1 cells after 24 hrs of infection, cDNA was synthesized for TLDA real time PCR reaction using TaqMan MicroRNA Reverse Transcription kit and Megaplex Human Pool A and Pool B stem loop RT primers (version 3.0) as per manufacturer’s protocol. Further real time reaction was performed on QuantStudio 12K Flex Real-Time PCR System (Applied Biosystems) by using cDNA (without pre-amplification) on TLDA card A and card B (version 3.0).

2017-04-20 | GSE94007 | GEO

10x single-cell RNAseq profiling of influenza Virus infected Human Lung Tissue Explants (HLTE)

Project description:This study investigates the interactions between Influenza A virus (IAV) and lung tissue at the single-cell level using the 10x Genomics single-cell RNA sequencing (scRNAseq) platform. Lung tissue samples from a donor with COPD were infected with IAV, and both infected and uninfected samples were analyzed 24 hours post-infection. Single-cell suspensions from multiple infected and uninfected tissue pieces were pooled and analyzed to identify cell types and their responses. A total of 10 cell types were identified, including various airway epithelial cells and immune cells. Apoptotic T cells, characterized by high mitochondrial DNA content, were excluded from gene expression analyses. The presence of viral mRNA was used to indicate viral transcription activity, although distinguishing between intracellular synthesis and extracellular uptake of viral mRNA remains challenging.

2024-05-29 | GSE268542 | GEO

Phosphate depletion: A novel trigger for Mycobacterium tuberculosis persistence

Project description:These data represent the global gene expression profile of Mycobacterium tuberculosis after 24 hrs and 72 hrs of inorganic phosphate starvation. Differentially regulated genes appear to include those encoding proteins involved in adaptation to phosphate starvation, namely those involved in phosphate regulation and phosphate assimilation, as well as those involved in the stringent response.

2009-09-22 | GSE14840 | GEO

Transcriptome analysis of MazF6 overexpression in Mycobacterium tuberculosis

Project description:We report the effects of MazF6 overexpression on Mycobacterium tuberculosis H37Rv strain. The total RNA was isolated from M. tuberculosis harboring either pTetR-int or pTetR-Int-mazF6 the expression was induced with 50 ng/ml anhydrotetracycline for 24 hrs.

2022-04-01 | GSE182591 | GEO

Genome wide expession analysis of mouse bone marrow derive macrophage (Bmdm) cell stimulated with cytokine and infected with mycobacterium tuberculosis

Project description:Bmdm cells were differentiated for 10 days and harvested and culture in six well plate followed by cytokine stimulation after 24 hrs cells were infected with mycobacterium tuberculosis to identify the host factors involved in infection.

2015-02-26 | GSE56736 | GEO

Celll type specific gene expression from healthy human lung tissue infected with mycobacterium tuberculosis (innate).

Project description:We have investigated the initial responses in human lung tissue explants to Mtb infection, focusing primarily on gene expression patterns in different tissue resident innate cell types

2018-06-04 | GSE112483 | GEO

Celll type specific gene expression from healthy human lung tissue infected with mycobacterium tuberculosis (ILC).

2018-06-04 | GSE112482 | GEO

Proteome of microparticles derived from Mycobacterium tuberculosis infected macrophages

Project description:Microparticles (MP) are small membranous particles (100-1000 nm) released under normal steady-state conditions and are thought to provide a communication network between host cells. Previous studies demonstrated that Mycobacterium tuberculosis (M.tb) infection of macrophages increased the release of MPs, and these MPs induced a proinflammatory response from uninfected macrophages in vitro and in vivo following their transfer into uninfected mice. To determine how M.tb infection modulates the protein composition of the MPs, and if this contributes to their proinflammatory properties, we compared the proteomes of MPs derived from M.tb-infected (TBinf-MP) and uninfected human THP-1 monocytic cells. MP proteins were analysed by GeLC-MS/MS with spectral counting revealing 68 proteins with statistically significant differential abundances. The 42 proteins increased in abundance in TBinf-MPs included proteins associated with immune function (7), lysosomal/endosomal maturation (4), vesicular formation (12), nucleosome proteins (4) and antigen processing (9). Prominent among these were the type I interferon inducible proteins, ISG15, IFIT1, IFIT2, and IFIT3. Exposure of uninfected THP-1 cells to TBinf-MPs induced increased gene expression of isg15, ifit1, ifit2, and ifit3 and the release of proinflammatory cytokines. These proteins may regulate the proinflammatory potential of the MPs and provide candidate biomarkers for M.tb infection.

2022-02-28 | PXD002026 | Pride

Differential production of small RNAs in Mycobacterium tuberculosis infected primary human monocyte-derived macrophages

Project description:Primary human monocytes were isolated from four healthy donors. Monocytes were differentiated into macrophages, infected with virulent Mycobacterium tuberculosis (Mtb), strain H37Rv, for 24 or 48 hours, at a multiplicity of infection of 5 or 10. Following infection, infected cells and time-matched uninfected controls were harvested, total RNA including small RNAs was isolated and used for next-generation small RNA sequencing. Small RNA sequencing data was processed using miRge2.0, including a novel miRge2.0-based tRF detection tool. Processed data was used to determine differential expression of microRNAs and differential production of tRNA-derived fragments (tRFs) during infection with Mtb.

2020-10-15 | GSE151050 | GEO

Pulse-chase SILAC of Mycobacterium tuberculosis infected THP-1 macrophages

Project description:Pulse chase SILAC was used to identify protein turnover within human macrophages infected with mycobacterium tuberculosis CDC1551, a ppe38-71 mutant strain, a complemented strain and an uninfected control.

2021-06-15 | PXD021166 | Pride

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data