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ATAC-seq reveals GATA-1- and heme-dependent chromatin targeting in erythroid cells.

ABSTRACT: CRISPR/Cas9-mediated ablation of two Alas2 intronic cis-elements in G1E-ER-GATA1 cells strongly reduced GATA-1-induced Alas2 transcription, heme biosynthesis, and GATA-1 regulation of other vital constituents of the erythroid cell transcriptome. Bypassing Alas2 function in Alas2 cis-element-mutant (double mutant) cells by providing its catalytic product 5-aminolevulinic acid (5-ALA) rescued heme biosynthesis and a subset of GATA-1-dependent genetic network. Using the same system, we discovered a GATA-1- and heme-dependent circuit that regulates chromatin accessibility during erythroid maturation.

ORGANISM(S): Mus musculus

PROVIDER: GSE114996 | GEO | 2018/05/30

REPOSITORIES: GEO

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GATA-1 and heme regulate the erythroid cell transcriptome.

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