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Transcriptome sequencing of differentially expressed genes of the skin tissue of Ncstn ΔKC mice and normal mice

ABSTRACT: We constructed keratinocyte-specific Ncstn-knockout mice to investigate the role of NCSTN in the pathogenesis of human AI.

ORGANISM(S): Mus musculus

PROVIDER: GSE115100 | GEO | 2018/05/31

REPOSITORIES: GEO

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miRNA expression signatures of the skin tissue of NcstnΔKC mice and normal mice

Project description:We constructed keratinocyte-specific Ncstn-knockout mice to investigate the role of NCSTN in the pathogenesis of human AI.

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transcriptome-wide map of NCSTN-KD and NC Hacat cells

Project description:Acne inversa (AI) is an inflammatory skin disease associated with the nicastrin (NCSTN) mutations. Family history with autosomal dominant inheritance has heen reported in AI patients and are associated with mutations in the γ-secretase subunit, nicastrin (NCSTN), presenilin enhancer 2 (PSENEN), and presenilin-1 (PSEN1). Among them, NCSTN gene has the highest mutation rate. To detect the impact of NCSTN deficiency on AI keratinocytes. Here, using the short hairpin RNA (shRNA)-mediated gene knockdown and RNA sequencing technology, we explored the differentially expressed genes regulated by NCSTN deficiency in HaCaT cells.

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Transcriptome sequencing of differentially expressed genes of the skin tissue of Ncstn ΔKC mice and normal mice

Project description:Transcriptome sequencing of differentially expressed genes of the skin tissue of Ncstn ΔKC mice and normal mice

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Project description:The present study was designed to use small interfering RNA (siRNA) approach to identify the effects of NCSTN silencing on human immortal keratinocyte cell line, HaCaT. The alteration of gene expression profiles after NCSTN gene silencing in HaCaT cells was investigated employing Agilent whole genome microarray

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Effects of nicastrin (NCSTN) siRNA knockdown in HEK293 and HEK001 cells.

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