Genomics

Dataset Information

0

Genome-wide maps of chromatin state and KAP1 occupancy in KRAB zinc finger protein (KRAB-ZFP) gene cluster KO ES cells and testis


ABSTRACT: We have shown that many mouse KRAB-ZFPs target retrotransposons which are generally marked by H3K9me3 and KAP1 in ES cells. To test whether these KRAB-ZFPs are require to establish and maintain repressive chromatin marks at these elements, we performed ChIP-seq with antibodies against KAP1 (2 KRAB-ZFP cluster KOs) and H3K9me3 (5 KRAB-ZFP cluster KOs). We show that KRAB-ZFP targeted retrotransposons show reduced KAP1 binding and H3K9me3 in these KO ES cells. Furthermore, we performed ChIP-seq with antibodies against histone modifications typical for promoters and enhancers in Chr4-cl KO ES cells and testis. We show that, in ES cells, ETn retrotransposons gain signatures of active enhancers which acts on earby gene expression. In testis, a handful of different retrotransposons gain enhancer marks, although to a lesser degree. In conclusion, our data shows that KRAB-ZFPs are required to maintain H3K9me3 at retrotransposons in ES cells and to prevent some retrotransposons of becoming enhancers that affect gene expression patterns.

ORGANISM(S): Mus musculus

PROVIDER: GSE115286 | GEO | 2019/06/27

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2016-03-15 | E-GEOD-74278 | biostudies-arrayexpress
2019-06-27 | GSE115288 | GEO
2019-06-27 | GSE115287 | GEO
2019-06-27 | GSE115289 | GEO
2016-03-15 | GSE74278 | GEO
2012-05-16 | E-GEOD-36850 | biostudies-arrayexpress
2012-12-16 | E-GEOD-38579 | biostudies-arrayexpress
2012-05-16 | E-GEOD-34446 | biostudies-arrayexpress
2024-10-31 | GSE208295 | GEO
2024-10-31 | GSE207017 | GEO