Methylation profiling

Dataset Information

0

Epigenetic optical mapping of 5-hydroxymethylcytosine in nanochannel arrays


ABSTRACT: We present a long-read, single-molecule mapping technology that generates hybrid genetic/epigenetic profiles of native chromosomal DNA. The genome-wide distribution of 5-hmC in human peripheral blood cells correlates well with 5-hmC DNA immunoprecipitation (hMeDIP) sequencing. However, the long single-molecule read-length of 100 kbp-1 Mbp produces 5-hmC profiles across variable genomic regions that failed to show up in the sequencing data. In addition, optical 5-hmC mapping shows a strong correlation between the 5-hmC density in gene bodies and the corresponding level of gene expression.

ORGANISM(S): Homo sapiens

PROVIDER: GSE115454 | GEO | 2018/06/08

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

| PRJNA475101 | ENA
2018-02-25 | GSE102473 | GEO
| PRJEB52551 | ENA
2015-02-03 | E-GEOD-47590 | biostudies-arrayexpress
2012-09-18 | GSE38231 | GEO
| EGAD00001007049 | EGA
2012-09-18 | E-GEOD-38231 | biostudies-arrayexpress
2011-04-13 | E-GEOD-24841 | biostudies-arrayexpress
2011-04-13 | GSE24841 | GEO
2014-12-17 | E-MTAB-3177 | biostudies-arrayexpress