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Determination of branch point (BP) usage by wild-type and mutant SF3B1 using a minigene library

ABSTRACT: We address define differential usage of branchpoint (BP) in by wild-type SF3B1 SF3B1-K700E using a synthetic mini-gene synthetic library with variable 3' spice sites (3'SS). Minigene-specific libraries were prepared and sequenced on the illumina platform to determine differnces in splice-site usage.

ORGANISM(S): Mus musculus synthetic construct Homo sapiens

PROVIDER: GSE115547 | GEO | 2018/10/30

REPOSITORIES: GEO

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