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GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE115745 | GEO | 2018/06/14

REPOSITORIES: GEO

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GATA3 Abundance Is a Critical Determinant of T Cell Receptor β Allelic Exclusion.

Project description:Allelic exclusion describes the essential immunological process by which feedback repression of sequential DNA rearrangements ensures that only one autosome expresses a functional T or B cell receptor. In wild-type mammals, approximately 60% of cells have recombined the DNA of one T cell receptor β (TCRβ) V-to-DJ-joined allele in a functional configuration, while the second allele has recombined only the DJ sequences; the other 40% of cells have recombined the V to the DJ segments on both alleles, with only one of the two alleles predicting a functional TCRβ protein. Here we report that the transgenic overexpression of GATA3 leads predominantly to biallelic TCRβ gene (Tcrb) recombination. We also found that wild-type immature thymocytes can be separated into distinct populations based on intracellular GATA3 expression and that GATA3LO cells had almost exclusively recombined only one Tcrb locus (that predicted a functional receptor sequence), while GATA3HI cells had uniformly recombined both Tcrb alleles (one predicting a functional and the other predicting a nonfunctional rearrangement). These data show that GATA3 abundance regulates the recombination propensity at the Tcrb locus and provide new mechanistic insight into the historic immunological conundrum for how Tcrb allelic exclusion is mediated.

| S-EPMC5452726 | biostudies-literature

GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion [GATA3 overexpression]

Project description:RNA-seq in DN3a stage thymocytes isolated from wild type and GATA3 overexpression Tg mouse

2018-06-14 | GSE115743 | GEO

GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion [GATA3-GFP]

Project description:RNA-seq in GFP-positive and GFP-negative DN4 stage thymocytes isolated from GATA3-GFP fusion protein knock-in mouse

2018-06-14 | GSE115740 | GEO

GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion

Project description:GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion

| PRJNA475904 | ENA

GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion [GATA3 overexpression]

Project description:GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion [GATA3 overexpression]

| PRJNA475906 | ENA

GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion [GATA3-GFP]

Project description:GATA3 abundance is a critical determinant of T cell receptor beta allelic exclusion [GATA3-GFP]

| PRJNA475908 | ENA

FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response

Project description:This SuperSeries is composed of the SubSeries listed below.

2010-11-17 | GSE25316 | GEO

Activator protein-2γ is a critical determinant of estrogen receptor interactome formation and gene transcription in breast cancer

Project description:This SuperSeries is composed of the SubSeries listed below.

2011-06-02 | GSE26741 | GEO

FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response

Project description:This SuperSeries is composed of the following subset Series: GSE25314: FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part I) GSE25315: FoxA1 is a critical determinant of Estrogen Receptor function and endocrine response (part II) Refer to individual Series

2010-11-17 | E-GEOD-25316 | biostudies-arrayexpress

Normal T lymphocytes can express two different T cell receptor beta chains: implications for the mechanism of allelic exclusion.

Project description:We have examined the extent of allelic exclusion at the T cell receptor (TCR) beta locus using monoclonal antibodies specific for V beta products. A small proportion (approximately 1%) of human peripheral blood T cells express two V beta as determined by flow cytometric analysis, isolation of representative clones, and sequencing of the corresponding V beta chains. Dual beta T cells are present in both the CD45R0+ and CD45R0- subset. These results indicate that dual beta expression is compatible with both central and peripheral selection. They also suggest that the substantial degree of TCR beta allelic exclusion is dependent only on asynchronous rearrangements at the beta locus, whereas the role of the pre-TCR is limited to signaling the presence of at least one functional beta protein.

| S-EPMC2191970 | biostudies-other

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