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Transcriptional profiling by 4SU-seq in mouse ESCs and ESC-derived neural progenitor cells.

ABSTRACT: Nascent RNA was metabolically labelled with 4SU in undifferentiated and ESC-derived neural progenitor cells (NPCs). 4SU incorporated RNA was isolated and deep-sequenced at day 0 (ESCs), 3, 5 and 7 of differentiation. NPC differentiation was monitored through expression of a GFP reporter insereted into the Sox1 locus (46C reporter ESC line; PMID: 12524553). The aim was to monitor changes in transcription as ESCs differentiate into NPCs and relate this to enhancer activity.

ORGANISM(S): Mus musculus

PROVIDER: GSE115774 | GEO | 2019/01/12

REPOSITORIES: GEO

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