Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Self-organization and symmetry breaking in intestinal organoid development

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Mus musculus

PROVIDER: GSE115958 | GEO | 2019/04/26

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Self-organization and symmetry breaking in intestinal organoid development.

Project description:Intestinal organoids are complex three-dimensional structures that mimic the cell-type composition and tissue organization of the intestine by recapitulating the self-organizing ability of cell populations derived from a single intestinal stem cell. Crucial in this process is a first symmetry-breaking event, in which only a fraction of identical cells in a symmetrical sphere differentiate into Paneth cells, which generate the stem-cell niche and lead to asymmetric structures such as the crypts and villi. Here we combine single-cell quantitative genomic and imaging approaches to characterize the development of intestinal organoids from single cells. We show that their development follows a regeneration process that is driven by transient activation of the transcriptional regulator YAP1. Cell-to-cell variability in YAP1, emerging in symmetrical spheres, initiates Notch and DLL1 activation, and drives the symmetry-breaking event and formation of the first Paneth cell. Our findings reveal how single cells exposed to a uniform growth-promoting environment have the intrinsic ability to generate emergent, self-organized behaviour that results in the formation of complex multicellular asymmetric structures.

| S-EPMC6544541 | biostudies-literature

Self-organization and symmetry breaking in intestinal organoid development [scRNA-Seq]

Project description:Intestinal organoids are complex three-dimensional structures that mimic cell type composition and tissue organization of the intestine by recapitulating the self-organizing capacity of cell populations derived from a single stem cell. Crucial in this process is a first symmetry-breaking event, in which only a fraction of identical cells in a symmetrical cyst differentiate into Paneth cells, which in turn generates the stem cell niche and leads to asymmetric structures such as crypts and villi. We here combine a quantitative single-cell gene expression and imaging approach to characterize the development of intestinal organoids from a single cell. We show that intestinal organoid development follows a regeneration process driven by transient Yap1 activation. Cell-to-cell variability in Yap1, emerging in symmetrical cysts, initiates a Notch/Dll1 lateral inhibition event driving the symmetry-breaking event and the formation of the first Paneth cell. Our findings reveal how single cells exposed to a uniform growth-promoting environment have the intrinsic ability to generate emergent, self-organized behavior resulting in the formation of complex multicellular asymmetric structures.

2019-04-25 | GSE115956 | GEO

Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq]

2019-04-25 | GSE115955 | GEO

Self-organization and symmetry breaking in intestinal organoid development

Project description:Self-organization and symmetry breaking in intestinal organoid development

| PRJNA476588 | ENA

Self-organization and symmetry breaking in intestinal organoid development [scRNA-Seq]

Project description:Self-organization and symmetry breaking in intestinal organoid development [scRNA-Seq]

| PRJNA476590 | ENA

Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq]

Project description:Self-organization and symmetry breaking in intestinal organoid development [bulk RNA-Seq]

| PRJNA476589 | ENA

Self-organization of Plk4 regulates symmetry breaking in centriole duplication.

Project description:During centriole duplication, a single daughter centriole is formed next to the mother centriole. The molecular mechanism that determines a single duplication site remains a long-standing question. Here, we show that intrinsic self-organization of Plk4 is implicated in symmetry breaking in the process of centriole duplication. We demonstrate that Plk4 has an ability to phase-separate into condensates via an intrinsically disordered linker and that the condensation properties of Plk4 are regulated by autophosphorylation. Consistently, the dissociation dynamics of centriolar Plk4 are controlled by autophosphorylation. We further found that autophosphorylated Plk4 is already distributed as a single focus around the mother centriole before the initiation of procentriole formation, and is subsequently targeted for STIL-HsSAS6 loading. Perturbation of Plk4 self-organization affects the asymmetry of centriolar Plk4 distribution and proper centriole duplication. Overall, we propose that the spatial pattern formation of Plk4 is a determinant of a single duplication site per mother centriole.

| S-EPMC6472344 | biostudies-literature

Symmetry breaking of tissue mechanics in wound induced hair follicle regeneration

Project description:This SuperSeries is composed of the SubSeries listed below.

2021-02-19 | GSE159939 | GEO

Stabilization of cell-cell adhesions prevents symmetry breaking and locks in pluripotency in 3D gastruloids

Project description:This SuperSeries is composed of the SubSeries listed below.

2022-09-23 | GSE199767 | GEO

Symmetry breaking in gastruloid development

Project description:We use single cell RNA sequencing to describe the transcriptional changes during gastruloid development from 0 to 120h hours.

2023-04-29 | GSE229513 | GEO