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Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence

ABSTRACT: Lysine acetylation is critical in regulating important biological processes in many organisms, yet little is known about acetylome evolution and its contribution to phenotypic diversity. Here, we compare the acetylomes of baker’s yeast and the three deadliest human fungal pathogens, Cryptococcus neoformans, Candida albicans, and Aspergillus fumigatus. Using mass spectrometry enriched for acetylated peptides together with public data from Saccharomyces cerevisiae, we show that fungal acetylomes are characterized by dramatic evolutionary dynamics and limited conservation in core biological processes. Notably, the levels of protein acetylation in pathogenic fungi correlate with their pathogenicity. Using gene knockouts and pathogenity assays in mice, we identify deacetylases with critical roles in virulence and protein translation elongation. Finally, through mutational analysis of deactylation motifs we find evidence of positive selection at specific acetylation motifs in fungal pathogens. These results shed new light on the pathogenicity regulation mechanisms underlying the evolution of fungal acetylomes.

ORGANISM(S): Cryptococcus neoformans var. grubii H99

PROVIDER: GSE116040 | GEO | 2019/04/26

REPOSITORIES: GEO

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In-depth evaluation of the acetylome in major human fungal pathogens elucidates the essential role of lysine acetylation in regulation of fungal pathogenicity

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Fungal acetylome comparative analysis identifies an essential role of acetylation in human fungal pathogen virulence

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