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Identification of a cancer driver lncRNA in DNA damage response

ABSTRACT: We report that the lncRNA SCAT7 (ELF3-AS1) is involved in the activation of the DNA damage response in cisplatin resistant LUAD cells, akin cisplatin in sensitive cells. Therefore SCAT7 is a potential therapeutic target for drug resistant cancers

ORGANISM(S): Homo sapiens

PROVIDER: GSE116192 | GEO | 2021/01/06

REPOSITORIES: GEO

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Knockdown of lncRNA ELF3-AS1 in two gastric cancer cell lines

Project description:Our data showed that ELF3-AS1 was a nuclear lncRNA, and its biofunction in cancers is largely unknown to date. To explore the function of lncRNA ELF3-AS1 in gastric cancer, loss-of-function study was performed in SGC7901 and AGS cell lines. RNA sequencing studies showed that the expression of almost all the histone coding genes were significantly increased after knocking down of ELF3-AS1 in SGC7901 and AGS cell lines.

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Identification of differentially expressed miRNAs after knockdown of ELF3-AS1 in gastric cancer

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Project description:Long noncoding RNAs (lncRNAs) are known to regulate the development and progression of various cancers, however, few lncRNAs have been well characterized in lung adenocarcinoma (LUAD). Understanding the expression profile of lncRNAs and protein-coding genes is critical to develop new diagnosis and treatment strategies for LUAD and improving the prognosis of diagnosed patients. Five female LUAD patients with no smoking history were selected to profile lncRNA and protein-coding gene expression with microarrays. Paired tumor tissues and adjacent nontumor tissues were collected and confirmed by pathologists.

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Knockdown of lncRNA ELF3-AS1 in two gastric cancer cell lines

Project description:Knockdown of lncRNA ELF3-AS1 in two gastric cancer cell lines

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HHIP-AS1 lncRNA promotes tumorigenicity in human SHH-driven brain tumors

Project description:Long non-coding RNAs (lncRNAs) comprise a diverse class of gene expression regulators with emerging roles in many biological processes including cancer. Here we show that the expression of the lncRNA Hedgehog Interacting Protein Antisense 1 (HHIP-AS1) is a hallmark feature of human SHH-driven tumors. Importantly, loss of HHIP-AS1 leads to reduced tumor growth in SHH-driven tumors in vitro and in vivo. Our results demonstrate the power of cross-entity transcriptome-wide comparisons to identify novel epigenetic–regulatory lncRNA circuitries underlying human cancers.

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DNA methylation profiling of lung adenocarcinomas and patient-matched non-malignant lung

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