Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Laminin-directed fate determination of human induced pluripotent stem cells (hiPSCs) during ocular cell differentiation

ABSTRACT: The extracellular matrix plays a crucial role in stem cell maintenance, expansion and differentiation. Laminin, a basement membrane protein, is effective in supporting cultures of human pluripotent stem cells. To furhter investigate the effects of different isoforms of laminin on ectodemral differentiation of hiPSCs, we performed gene expression analysis of differentiated human iPS cells on E8 fragments of laminin isoforms (LN111E8, LN211E8, LN332E8, LN411E8, and LN511E8).

ORGANISM(S): Homo sapiens

PROVIDER: GSE116591 | GEO | 2018/10/17

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomic signature of treatment with recombinant laminin 511-E8 and acetazolamide in human H69 cholangiocytes

Project description:IgG4-related cholangitis (IRC) is the hepatobiliary manifestation of IgG4-related disease (IgG4-RD), a systemic, B-cell driven, fibroinflammatory disorder. To date, numerous autoantigens have been described including laminin 511-E8 and carbonic anhydrase (CA) enzymes (PMID 30089633, 15647194). Laminin 511-E8 is an extracellular matrix protein that has been shown to upregulate secretory components and aid in the cholangiocyte differentiation of induced pluripotent stem cells (PMID 27103433). Carbonic anhydrase enzymes catalyse the reversible hydration of carbon dioxide, thereby producing bicarbonate and a single proton. In humans there are 14 different CA isoforms with a confined subcellular distribution. All isoforms can be targeted by the pan-CA inhibitor acetazolamide, which is a registered drug for multiple indications. We aimed to investigate the role of IgG4-RD autoantigens laminin 511-E8 and CA enzymes in human cholangiocytes. Transcriptional profiling may provide insights into the underlying molecular mechanisms of these proteins on the cholangiocellular level. Thus, by RNA sequencing the transcriptomic signature of recombinant laminin 511-E8 and acetazolamide treatment was assessed in human H69 cholangiocytes.

2024-02-15 | GSE221746 | GEO

Laminin-directed fate determination of human induced pluripotent stem cells (hiPSCs) during ocular cell differentiation

Project description:Laminin-directed fate determination of human induced pluripotent stem cells (hiPSCs) during ocular cell differentiation

| PRJNA479465 | ENA

Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells [Bulk RNA-Seq]

Project description:Obtaining highly purified differentiated cells via directed differentiation from human pluripotent stem cells (hPSCs) is an essential step for the clinical application of hPSCs. Among the various conditions that should be optimized, the precise role and contribution of the extracellular matrix (ECM) during differentiation are relatively unclear. Here, using a short fragment of laminin 411 (LM411-E8), an ECM predominantly expressed in the vascular endothelial basement membrane, we demonstrated that the directed switching of defined ECMs robustly yields highly purified (>95%) endothelial progenitor cells (PSC-EPCs) from hPSCs in integrin-laminin axis and rho signaling pathway-dependent manner.

2016-11-08 | GSE85782 | GEO

Laminin-guided highly efficient endothelial commitment from human pluripotent stem cells [Single Cell RNA-Seq]

2016-11-08 | GSE85783 | GEO

Effect of laminin adhesion on basal human mammary epithelial cells

Project description:Mammary epithelium is composed by luminal and basal epithelial cells, which are adhere to the basement membrane (BM). To dissect how basal cell functions are regulated by BM laminin adhesion, we performed RNA sequencing of basal human mammary epithelial cells adhered on laminin-111, -421 or -521 coated cell culture plates for 48 hours.

2022-11-07 | GSE197950 | GEO

Loss of Laminin α3 induces cell invasion and macrophage infiltration in cutaneous squamous cell carcinoma

Project description:Cutaneous squamous cell carcinoma (cSCC) is a common cancer which invades into dermis through the basement membrane. Laminins are basement membrane proteins which are copiously secreted by SCC keratinocytes. Here we provide evidence using in vitro and in vivo 3D models as well as human tumors that the loss of the laminin a3 chain observed in poorly differentiated cSCC increases tumor invasion with an amoeboid phenotype. There is an increase in recruitment and differentiation of TAMs around invading tumor with a shift towards M2 TAMs. These data provide evidence that loss of the laminin a3 chain in cSCC has an effect on both the epithelial and immune components of cSCC resulting in an aggressive tumor microenvironment.

2020-12-01 | GSE123438 | GEO

Knockdown of laminin alpha 5 chain in colorectal cancer cell line HT-29

Project description:Laminin-mediated signaling via membrane receptors actively participate in most normal biological process and pathologies. Laminins involve in all step of metastatic cascade including tumor cell proliferation, migration, anoikis resistance, extravasation, epithelial to mesenchymal transition etc. The 5-containing laminins (LAMA5) are one of the three types of laminins predominantly expressed in the epithelial basement membrane in the adult intestine as well as during crypt-villus development. Likewise laminins 511 and 521 (laminin-10 and laminin-11) have been shown as important matrix component to facilitate stem cell survival and to maintain their stem cell properties. Thus, it is assuming that laminins play a role in the formation of premetastatic niches. To investigate the role of 5-containing laminins in colorectal cancer we performed the knockdown of LAMA5 gene expression in HT-29 cell line. Here we present the results of comprehensive gene expression analysis using Affymetrix microarrays in cells transduced by shRNA to LAMA5, control nonsense shRNA, and parental cells as well.

2019-03-18 | E-MTAB-7754 | biostudies-arrayexpress

Klinefelter syndrome derived hiPSCs show similar XCI behavior as female hPSCs

Project description:In this study, we reprogrammed fibroblasts from two azoospermic Klinefelter syndrome (KS) patients, and two healthy male and one healthy female donor with an efficient integration-free method using episomal plasmids and laminin-521 (LN521). Whole genome transcriptomics analysis showed differentially expressed genes between KS and healthy male donors with enrichment in gene ontology (GO) terms associated with fertility, cardiovascular development, ossification and brain development, for both KS hiPSCs and fibroblasts, correlating with the KS clinical phenotype. Thorough XCI analysis combining transcriptomics data, RNA FISH and H3K27me3 staining, revealed skewed XCI in one KS fibroblast line and variability in XCI state of KS hiPSCs similar to female hiPSCs, showing either XaXi or XaXe status. Furthermore, we found up-regulated X-linked genes involved in nervous system development, synaptic transmission as well as metabolic processes supporting the potential use of KS derived hiPSC as an in vitro model for KS.

2019-10-05 | E-MTAB-7340 | biostudies-arrayexpress

RNA-seq of hiPSCs adapted to homemade weekend-free media

Project description:Homemade weekend free media are a promising way for the labs to reduce the manpower and high costs associated with the culture of human induced pluripotent stem cells (hiPSCs) in feeder-free conditions. However, as these media would have a different concentration of various growth factors, cells may perceive a change in the signalling environments and change their transcription profile. To investigate the extent of this change, we decided to perform RNA sequencing and study the change of gene expression as compared to cells grown in commercial, daily changed E8.

2024-07-11 | E-MTAB-14237 | biostudies-arrayexpress

Laminin-binding integrins are essential for the maintenance of functional secretory epithelium in lactation

Project description:Integrin dimers α3/β1, α6/β1 and α6/β4 are the mammary epithelial cell receptors for laminins, which are major components of the basement membrane, a specialized extracellular matrix surrounding the mammary epithelium. The roles of specific basement membrane components and their integrin receptors in the regulation of functional gland development have not been analyzed in detail. To investigate the functions of laminin-binding integrins, we obtained mutant mice with mammary luminal cell-specific deficiencies of the α3 and α6 integrin chains generated by the Cre-Lox approach. During pregnancy, mutant mice displayed low levels of luminal progenitor activity and retarded lobulo-alveolar development, whereas their mammary glands seemed to be functional at the onset of lactation. Myoepithelial cell morphology was markedly altered in mutant glands, suggesting cellular compensation mechanisms involving cytoskeleton reorganization. However, lactation was not sustained in mutant mice, and the glands underwent precocious involution. Inactivation of the p53 gene rescued the growth defects but did not restore lactogenesis in mutant mice. This study reveals an essential role for laminin-binding integrins in functional mammary gland development.

2020-02-04 | GSE132281 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data