Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Sinorhizobium meliloti root nodule bacterial transcriptomes for wild type and four mutant strains.

ABSTRACT: We characterized transcriptomes of Sinorhizobium meliloti root nodule bacteria with mutations in sigma factor genes.

ORGANISM(S): Medicago truncatula Sinorhizobium meliloti

PROVIDER: GSE116664 | GEO | 2018/09/05

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Identification of RpoE2 target genes in Sinorhizobium meliloti

2013-07-22 | GSE40391 | GEO

NODULE INCEPTION recruits the lateral root developmental program for symbiotic nodule organogenesis

Project description:In order to better understand the commonalities and differences in lateral root and nodule development, we compared their organogenesis and correlated this with changes in gene expression. To initiate lateral roots in Medicago truncatula we turned 2-day-old seedlings 135°, before returning them to their original axis of growth, while for nodule initiation we applied droplets of Sinorhizobium meliloti on the susceptibility zone of the root.

2019-09-20 | GSE133612 | GEO

Overexpression of Sinorhizobium meliloti ECF sigma factors.

Project description:We characterized transcriptomes for strains overexpressing each of the Sinorhizobium meliloti ECF sigma factors the via a plasmid-borne, melibiose-inducible promoter plasmid (PmelA; pCAP11: Pinedo et al. 2008 J Bacteriol 190:2947-2956) compared to control strains carrying the empty vector.

2018-09-05 | GSE116665 | GEO

Sinorhizobium meliloti root nodule bacterial transcriptomes for wild type and four mutant strains.

Project description:Sinorhizobium meliloti root nodule bacterial transcriptomes for wild type and four mutant strains.

| PRJNA479835 | ENA

Dual RpoH sigma factors in Sinorhizobium meliloti

Project description:Sinorhizobium meliloti can live as a soil saprophyte, and can engage in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including two putative RpoH (heat shock) sigmas. We used custom Affymetrix Symbiosis Chips to characterize the global transcriptional response of S. meliloti rpoH1, rpoH2 and rpoH1 rpoH2 mutants during heat shock and stationary phase growth. Under these conditions, expression of over 300 genes is dependent on rpoH1 and rpoH2.

2012-10-02 | GSE36186 | GEO

Host plant peptides elicit a transcriptional response to control the Sinorhizobium meliloti cell cycle during symbiosis

Project description:We profiled gene expression in synchronized cultures of Sinorhizobium meliloti treated with or without the nodule-specific cysteine-rich peptide 247. We compared gene expression between the two conditions at 5 timepoints (15, 30, 60, 90, 120) following the addition of the peptide

2014-01-21 | GSE54244 | GEO

Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [CtrA]

Project description:We characterized transcriptomes of a Sinorhizobium meliloti wild type strain (CL150) expressing either Ca. Liberibacter asiaticus ctrA or Sinorhizobium meliloti ctrA

2019-08-01 | GSE124977 | GEO

Identifying the regulon of the Sinorhizobium meliloti JspA protease

Project description:Sinorhizobium meliloti establishes symbiotic relationship with compatible leguminous plants by inducing root nodule formation, colonizing such nodules, and fixing molecular nitrogen for the host in exchange for carbon compounds. This mutualistic process requires complex communication and tight regulation to allow yet constrain infection to specific tissues. Production of succinoglycan, or exopolysaccharide-I (EPS-I), enables S. meliloti to invade the root cortex via infection threads. A previous genetic screen identified jspA (SMc03872), encoding an extracytoplasmic protease, as a regulator of EPS-I production. To elucidate its molecular role, we performed transcriptome analyses of strains overexpressing wild-type or mutant alleles of jspA. We observed changes in gene expression suggesting that JspA contributes to symbiosis efficiency by modulating the critical ExoR-ExoS-ChvI signaling pathway.

2023-05-10 | GSE155833 | GEO

Sinorhizobium meliloti as a model system to study Ca. Liberibacter asiaticus regulators. [RpoH]

Project description:We characterized transcriptomes of a Sinorhizobium meliloti rpoH1rpoH2 deletion mutant (RFF231; Lang et al. 2018, mSphere 3:e00454-18) expressing either Ca. Liberibacter asiaticus rpoH or Sinorhizobium meliloti rpoH1

2019-08-01 | GSE124982 | GEO

Identification of RpoE2 target genes in Sinorhizobium meliloti

Project description:Sinorhizobium meliloti lives as a soil saprophyte, and engages in a nitrogen fixing symbiosis with plant roots. To succeed in such diverse environments, the bacteria must continually adjust gene expression. Transcriptional plasticity in eubacteria is often mediated by alternative sigma factors interacting with core RNA polymerase. The S. meliloti genome encodes 14 of these alternative sigmas, including 11 extracytoplasmic function (ECF) sigmas. We used custom Affymetrix Symbiosis Chips to characterize the global transcriptional response of S. meliloti overexpressing the ECF sigma factor, RpoE2. Our work identifies over 200 genes whose expression is dependent on RpoE2. The gene expression profile of Sinorhizobium meliloti strain CL150 carrying the rpoE2 gene on a plasmid, where it was expressed from a melibiose-inducible promoter (the S. meliloti PmelA promoter), was compared to the gene expression profile of CL150 carrying the corresponding empty vector (pCAP11). CL150 is an Rm1021 strain that has been corrected for two point mutations: it is wild type for ecfR1 and pstC.

2013-07-22 | E-GEOD-40391 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data