Genomics

Dataset Information

0

Aging alters the epigenetic asymmetry of HSC division [scATAC-Seq]


ABSTRACT: Hematopoietic stem cells (HSCs) balance self-renewal and differentiation to maintain homeostasis. With aging, the frequency of polar HSCs decreases. Cell polarity in HSCs is controlled by the activity of the small RhoGTPase Cdc42. Here we demonstrate, using a comprehensive set of paired daughter cell analyses that include single cell 3D-confocal imaging, single cell transplants, single cell RNA-seq as well as single cell ATAC-seq, that the outcome of HSC divisions is strongly linked to the polarity status before mitosis, which is in turn determined by the level of the activity Cdc42 in stem cells. Aged apolar HSCs undergo preferentially self-renewing symmetric divisions, resulting in daughter stem cells with reduced regenerative capacity and lymphoid potential, while young polar HSCs undergo preferentially asymmetric divisions. Mathematical modeling in combination with experimental data implies a mechanistic role of the asymmetric sorting of Cdc42 in determining the potential of daughter cells via epigenetic mechanisms. Therefore, molecules that control HSC polarity might serve as modulators of the mode of stem cell division regulating the potential of daughter cells.

ORGANISM(S): Mus musculus

PROVIDER: GSE116707 | GEO | 2018/12/04

REPOSITORIES: GEO

Dataset's files

Source:
Action DRS
Other
Items per page:
1 - 1 of 1

Similar Datasets

2018-12-04 | GSE116708 | GEO
2020-09-18 | GSE158140 | GEO
2024-03-21 | PXD050287 | Pride
2018-09-05 | GSE119466 | GEO
2009-08-07 | GSE16516 | GEO
2021-10-05 | GSE165986 | GEO
2024-07-02 | PXD051560 | Pride
2021-10-23 | GSE166736 | GEO
2019-07-25 | GSE132357 | GEO
2022-08-15 | GSE167317 | GEO