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RNA-seq profiling of parental and NLUCAT1-deleted A549 cells under hypoxia

ABSTRACT: Parental or NLUCAT1 CRISPR/Cas9-deleted A549 clones (WT A549 Hx, n = 1 clone; DEL A549 Hx, n = 1 clone) were exposed to hypoxia (Hx) at 1% O2 for 24 hours. Libraries were generated from 500 ng of total RNAs using TruSeq Stranded Total RNA kit with Ribo-Zero (Illumina) and sequenced on a NextSeq500 sequencer (Illumina) with 2x75bp paired-end chemistry. Reads were aligned to the human genome release hg19 using STAR v2.4.0a with default parameters.

ORGANISM(S): Homo sapiens

PROVIDER: GSE117044 | GEO | 2019/09/04

REPOSITORIES: GEO

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