Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (polysome profiling)

ABSTRACT: Performed polysome profiling in cell lysates from two independent UBAP2L knockout clonal isolates and from two control samples.

ORGANISM(S): Homo sapiens

PROVIDER: GSE117291 | GEO | 2020/08/18

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (polysome profiling)

Project description:Large-scale tethered function assays identify factors that regulate mRNA stability and translation (polysome profiling)

| PRJNA482134 | ENA

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (HEK293T_RNA-seq)

Project description:we either depleted RBPs by lentiviral transduction of short-hairpin RNAs (shRNAs) or ectopically expressed the open reading frame of the protein. For each RBP, we performed two independent transductions of two different targeting shRNAs and two independent transductions of a non-targeting shRNA, or performed two independent transfections with a plasmid directing expression of the RBP as a V5-tagged fusion, with the FLAG construct as a control.

2020-08-18 | GSE117293 | GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation

Project description:This SuperSeries is composed of the SubSeries listed below.

2020-08-18 | GSE117294 | GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (eCLIP)

Project description:Enhanced UV crosslinking and immunoprecipitation (eCLIP) identifies thousands of endogenous mRNA targets for these prioritized RBPs that respond to protein level changes reminiscent of their effects in tethered functional assay.

2020-08-18 | GSE117290 | GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation.

Project description:The molecular functions of the majority of RNA-binding proteins (RBPs) remain unclear, highlighting a major bottleneck to a full understanding of gene expression regulation. Here, we develop a plasmid resource of 690 human RBPs that we subject to luciferase-based 3'-untranslated-region tethered function assays to pinpoint RBPs that regulate RNA stability or translation. Enhanced UV-cross-linking and immunoprecipitation of these RBPs identifies thousands of endogenous mRNA targets that respond to changes in RBP level, recapitulating effects observed in tethered function assays. Among these RBPs, the ubiquitin-associated protein 2-like (UBAP2L) protein interacts with RNA via its RGG domain and cross-links to mRNA and rRNA. Fusion of UBAP2L to RNA-targeting CRISPR-Cas9 demonstrates programmable translational enhancement. Polysome profiling indicates that UBAP2L promotes translation of target mRNAs, particularly global regulators of translation. Our tethering survey allows rapid assignment of the molecular activity of proteins, such as UBAP2L, to specific steps of mRNA metabolism.

| S-EPMC8221285 | biostudies-literature

Polysome profiling and ribosome footprinting of Drosophila mature oocyte and activated egg

Project description:We use mRNA-seq in combination with polysome profiling to determine translational status for all mRNAs in Drosophila mature oocytes and activated eggs. Puromycin-treated lysates are used as a negative control in polysome profiling experiments. Additionally, we use ribosome footprinting to globally measure translational efficiency of mRNAs in wild type mature oocytes as well as wild type and png mutant activated eggs.

2014-06-12 | GSE52799 | GEO

Large-scale tethered function assays identify factors that regulate mRNA stability and translation

Project description:Large-scale tethered function assays identify factors that regulate mRNA stability and translation

| PRJNA482133 | ENA

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (eCLIP)

Project description:Large-scale tethered function assays identify factors that regulate mRNA stability and translation (eCLIP)

| PRJNA482135 | ENA

Identification of polysome-associated translational regulators during oxidative stress and amino acid starvation

Project description:To identify RBPs with previously unappreciated roles in translational regulation, we performed polysome proteomics (polysome profiling and label-free mass spectrometry) to identify and quantify proteins associated with translating ribosomes in unstressed yeast cells and changes in association during two acute stresses that induce the integrated stress response (ISR): oxidative stress (0.45 mM H2O2 treatment for 15 min) and amino acid starvation (10 mM 3-AT treatment for 15 min). Five fractions were isolated from sucrose gradients prepared from each extract, corresponding to monosomes (F1) and polysomes with increasing numbers of ribosomes per mRNA (F2-F5). Unfractionated cyotplasmic lysates (totals) were analysed for each sample. Four replicate extracts were prepared and analysed for each condition.

2022-06-13 | PXD027903 | Pride

Large-scale tethered function assays identify factors that regulate mRNA stability and translation (HEK293T_RNA-seq)

Project description:Large-scale tethered function assays identify factors that regulate mRNA stability and translation (HEK293T_RNA-seq)

| PRJNA482136 | ENA