Comparison of PPIL3 knockdown in HEK293T cells compared to scrambled shRNA control
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ABSTRACT: HEK293T cells transduced with shRNA from MISSION library TRCN0000000185 using lentiviral delivery system. HEK293T cells transduced with scrambled shRNA, gifted from Dr. Mauricio Reginato. Tara L Davis, S. RaElle Jackson, Beth Adams, Anh Trinh, Jonathan Amora, Peter Naranjo, Gueil Wong-Shing, and Nii Martey performed primary experimental contributions to cell lines, RNA/cDNA preparation, and validation, all Drexel University College of Medicine, Philadelphia, PA. Hetty Rodriguez and John Tobias performed Bioanalyzer and microarray expreriments, and initial data processing. Affiliation: Molecular Profiling Facility and Genomic Analysis Core Bioinformatics Group, University of Pennsylvania, Philadelphia, PA. Human PPIL3 is a cyclophilin, an enzyme that interconverts cis and trans isomers of proline. PPIL3 associates with the human spliceosome, the complex and dynamic machinery that removes intronic sequence from pre-messenger RNA (pre-mRNA). Nothing is known about the function of PPIL3 in the nucleus. To understand the function of PPIL3, we knocked down PPIL3 in human cells. We characterized a set of alternative splicing and transcriptional events that are PPIL3-responsive. We used these splicing and transcriptional bioassays to show that PPIL3-responsive events are largely specific, even within the cyclophilin family.
ORGANISM(S): Homo sapiens
PROVIDER: GSE117302 | GEO | 2018/10/01
REPOSITORIES: GEO
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