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Repressive mark H3K27me3 in PRMT6 KO NT2/D1 cells

ABSTRACT: Protein arginine methyltransferase 6 (PRMT6) is an epigenetic regulator of fundamental cellular processes, such as gene expression and DNA repair. Asymmetric dimethylation of histone H3 at arginine 2 (H3R2me2a) is the major histone modification catalyzed by PRMT6. To identify the genome-wide deposition and transcriptional impact of H3R2me2a, we established PRMT6 deletion in a human cell model of neural differentiation. These knockout cells show severe neural differentiation defects. ChIP-seq profiling reveals that H3R2me2a is present at promoter as well as non-promoter sites in a PRMT6-dependent manner. Loss of H3R2me2a causes enhanced H3K4me3 deposition and target gene transcription supporting a genome-wide repressive nature of H3R2me2a. Intriguingly, the non-promoter H3R2me2a peaks co-localize with active enhancer marks, such as H3K4me1 and H3K27ac.

ORGANISM(S): Homo sapiens

PROVIDER: GSE117341 | GEO | 2018/09/27

REPOSITORIES: GEO

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Genomic occurrence analysis of PRMT6-dependent H3R2me2a

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Project description:Repressive mark H3K27me3 in PRMT6 KO NT2/D1 cells

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