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Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (macrophages)

ABSTRACT: Here we amplified a region in exon 5 of Ptgs2 that contains SNPs to allow us to differenitate between the C57 allele and the MOLF allele

ORGANISM(S): Mus musculus

PROVIDER: GSE117377 | GEO | 2018/12/19

REPOSITORIES: GEO

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Genetic Models Reveal cis and trans Immune-Regulatory Activities for lincRNA-Cox2.

Project description:An inducible gene expression program is a hallmark of the host inflammatory response. Recently, long intergenic non-coding RNAs (lincRNAs) have been shown to regulate the magnitude, duration, and resolution of these responses. Among these is lincRNA-Cox2, a dynamically regulated gene that broadly controls immune gene expression. To evaluate the in vivo functions of this lincRNA, we characterized multiple models of lincRNA-Cox2-deficient mice. LincRNA-Cox2-deficient macrophages and murine tissues had altered expression of inflammatory genes. Transcriptomic studies from various tissues revealed that deletion of the lincRNA-Cox2 locus also strongly impaired the basal and inducible expression of the neighboring gene prostaglandin-endoperoxide synthase (Ptgs2), encoding cyclooxygenase-2, a key enzyme in the prostaglandin biosynthesis pathway. By utilizing different genetic manipulations in vitro and in vivo, we found that lincRNA-Cox2 functions through an enhancer RNA mechanism to regulate Ptgs2. More importantly, lincRNA-Cox2 also functions in trans, independently of Ptgs2, to regulate critical innate immune genes in vivo.

| S-EPMC6291222 | biostudies-literature

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo

Project description:This SuperSeries is composed of the SubSeries listed below.

2018-12-19 | GSE117379 | GEO

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs)

Project description:Here we compare gene expression profiles between lungs taken from a Wild type mouse to lungs from a lincRNA-Cox2 knockout mouse The aim of the experiment is to determine what impact lincRNA-Cox2 has on gene expression within the lung

2018-12-19 | GSE105068 | GEO

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs and spleens)

Project description:Here we compare gene expression profiles between lungs and spleens following LPS in vivo injection into Wild type, lincRNA-Cox2 knockout and lincRNA-Cox2 mutant mice The aim of the experiment is to determine the cis and trans functions for lincRNA-Cox2 following in vivo LPS challenge within the spleen and lung

2018-12-19 | GSE116354 | GEO

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo

Project description:Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo

| PRJNA481951 | ENA

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (macrophages)

Project description:Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (macrophages)

| PRJNA481949 | ENA

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs)

Project description:Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs)

| PRJNA414568 | ENA

Developing high throughput single-cell single-nucleotide in situ pairwise sequencing for macromolecule spatail-omics

Project description:To identified the method which can differentiate in situ SNP modification, we constructed an RNAseq library to identify SNPs in the C6 cell line and parent-specific SNPs in the PVN of C57. Design probes for these SNPs for in situ SNP detection and was applied to detect tumor driver genes mutation and allele specific expression of parental genes.

2023-12-01 | GSE190219 | GEO

Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs and spleens)

Project description:Genetic models reveal cis and trans immune-regulatory activities for lincRNA-Cox2 in vivo (lungs and spleens)

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lincRNA-Cox2 regulates NLRP3 inflammasome and autophagy mediated neuroinflammation.

Project description:Inflammasome activation plays key roles in host defense, but also contributes to the pathogenesis of auto-inflammatory, and neurodegenerative diseases. As autophagy is connected with both the innate and adaptive immune systems, autophagic dysfunction is also closely related to inflammation, infection, and neurodegeneration. Here we identify that lincRNA-Cox2, previously known as a mediator of both the activation and repression of immune genes expression in innate immune cells, could bind NF-κB p65 and promote its nuclear translocation and transcription, modulating the expression of inflammasome sensor NLRP3 and adaptor ASC. Knockdown of lincRNA-Cox2 inhibited the inflammasome activation and prevented the lincRNA-Cox2-triggered caspase-1 activation, leading to decreased IL-1β secretion and weakened TIR-domain-containing adapter-inducing interferon-β (TRIF) cleavage, thereby enhancing TRIF-mediated autophagy. Elucidation of the link between lincRNA-Cox2 and the inflammasome-autophagy crosstalk in macrophage and microglia reveals a role for lncRNAs in activation of NLRP3 inflammasome and autophagy, and provides new opportunities for therapeutic intervention in neuroinflammation-dependent diseases.

| S-EPMC6294802 | biostudies-other

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