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ASH-1-catalyzed H3K36 methylation drives gene repression and marks H3K27me2/3-competent chromatin

ABSTRACT: ASH-1 orthologs are H3K36-specific methyltransferases that are conserved from fungi to humans but are poorly understood, in part because they are typically essential for viability. Here we examine the H3K36 methylation pathway of Neurospora crassa, which we find has just two H3K36 methyltransferases, ASH-1 and RNA polymerase II-associated SET-2. Our investigation of the interplay between SET-2 and ASH-1 uncovered a regulatory mechanism connecting ASH-1-catalyzed H3K36 methylation to repression of poorly transcribed genes. Our findings provide new insight into ASH-1 function, H3K27me2/3 establishment, and repression at facultative heterochromatin.

ORGANISM(S): Neurospora crassa

PROVIDER: GSE118495 | GEO | 2018/08/14

REPOSITORIES: GEO

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