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Dissecting polymerase pausing with TV-PRO-seq

ABSTRACT: Transcription of many genes in metazoans is subject to polymerase pausing, which corresponds to the transient arrest of transcriptionally engaged polymerase. It occurs mainly at promoter proximal regions and is not well understood. In particular, a genome-wide measurement of pausing times at high resolution has been lacking. This is because relevant experimental techniques are mostly based on average polymerase occupancies across many cells which cannot discriminate between few slow polymerases and many quick polymerases at a position, since the data corresponds to the average of many cells. We present here an extension of the PRO-seq assay, which we termed time variant PRO-seq (TV-PRO-seq)based on a detailed analysis of the logics underlying PRO-seq, that achieves this goal.

ORGANISM(S): Homo sapiens

PROVIDER: GSE118957 | GEO | 2019/08/01

REPOSITORIES: GEO

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Dissecting polymerase pausing with TV-PRO-seq

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