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Mapping the differential distribution of the cohesin subunit Scc1 in Trypanosoma brucei brucei.

ABSTRACT: Chromatin immunoprecipitation of genomic loci in Trypanosoma brucei where Scc1 is deposited. This was achieved by deletion of one Scc1 allele and N-terminal tagging of the second Scc1 allele with a Ty1-tag. During the ChIP experiment, the DNA was crosslinked and cells were permeabilized with digitonin. DNA was fragmented by sonication with a Covaris instrument. DNA bound to Ty1-Scc1 was pulled down by using a BB2 anti-Ty1 antibody. Cross links are reversed and the DNA was purified and prepared for Illumina sequencing.

ORGANISM(S): Trypanosoma brucei brucei

PROVIDER: GSE119071 | GEO | 2018/11/06

REPOSITORIES: GEO

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