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Transcriptome-wide analysis of TRIM21-mediated changes in gene expression after challenge with virus and antibody

ABSTRACT: We report that TRIM21 plays a crucial role in mediating antibody interference of adenovirus gene delivery and simultaneously initiates a transcriptional programme of immune genes.

ORGANISM(S): Mus musculus

PROVIDER: GSE119119 | GEO | 2018/09/17

REPOSITORIES: GEO

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Effect of Trim21 knockdown on MAPK and ERBB signaling pathways

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Project description:Macrophage polarization followed by acute myocardial infarction (MI) is essential for the regulation of inflammation and scar formation. Tripartite motif-containing protein 21 (TRIM21), a member of E3 ubiquitin ligases, is a crucial mediator in the process of inflammation and heart failure. However, the potential roles of TRIM21 in modulating post-MI inflammation and macrophage polarization remain elusive. We detected that the levels of TRIM21 were significantly reduced in macrophages of WT mice after MI. In contrast, MI was ameliorated in TRIM21 knockout (TRIM21-/-) mice with improved cardiac remodeling, characterized by a marked decrease in mortality, increased wall thickness, and improved cardiac function in comparison with wild-type (WT) MI mice. Importantly, TRIM21 deficiency decreased the post-MI apoptosis and DNA damage in the hearts of mice, and the accumulation of M1 phenotype macrophages in infarcted hearts significantly decreased in TRIM21-/- mice compared with WT controls. Mechanistically, depletion of TRIM21 orchestrated the process of M1 macrophage polarization via a PI3K/Akt signaling pathway. Overall, these data reveal that TRIM21 drives the inflammatory response and cardiac remodeling after MI via stimulating M1 macrophage polarization through a PI3K/Akt signaling pathway.

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TRIM21 enhances bortezomib sensitivity in multiple myeloma by halting pro-survival autophagy

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Transcriptome-wide analysis of TRIM21-mediated changes in gene expression after challenge with virus and antibody

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TRIM21 knockout mouse livers show enhanced antioxidant capacity and decreased compensatory proliferation

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TRIM21 innate immune detection of GBP1-positive Toxoplasma gondii is essential for host survival

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