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Genetic interaction mapping (GIM) for factors involved in RNA and protein synthesis and degradation

ABSTRACT: A S. cerevisiae strain a gene of interest mutated or deleted was combined with two collections of yeast mutants: one in which non-essential genes were deleted and one in which an long 3' UTR extension has been added to the mRNA of essential genes (GIM method, Decourty et al., 2008). Combined double-mutant deletion cells growth was quantified using barcodes that are specific for each gene mutation. A reference population was obtained by mixing the results of 15 GIM screens DNA prior to barcode amplification and labeling (Decourty et al., in preparation). The identification of genetic interactions between genes involved in different cellular pathways indicate new functions for previously characterized genes and new links between

ORGANISM(S): Saccharomyces cerevisiae S288C Saccharomyces cerevisiae

PROVIDER: GSE119174 | GEO | 2019/12/31

REPOSITORIES: GEO

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