Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Global Gene Expression of H9 hESC with different passage numbers

ABSTRACT: Transcriptome profile was obtained from a set of human embryonic stem cell (hESCs) line (WA09: H9) with different passage numbers (P1: 40s, P2: 100s, P3: 200s, P4: 300s passage). Culture adaptation occurs in hESCs during repeated in vitro culture to acquire ‘survival advantage’ to be highly resistant to various stresses. In special, difference in gene expression profile of cell death or apoptotic gene signature was evident between P1/P2 and P3/P4 hESCs.

ORGANISM(S): Homo sapiens

PROVIDER: GSE119386 | GEO | 2018/09/04

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Discovery of progenitor signatures by time series synexpression analysis during Drosophila cell immortalization [RNA-Seq]

Project description:To characterize the sequence of events associated with RasV12 immortalization of Drosophila embryonic cells, we generated transcriptional time series during cell line establishment, from primary cultures until passage (P) 19. We generated three transcriptional time series from three cell lines (R1, R4 and R5) by sampling the cultures at successive stages, early (P2-4), intermediate (P4-11), and late (P16-19), characterized by different passage times. Time points for the R1 time-series were: P2, P3, P4, P5, P7, P8, P10, P11, P16, P17 and P19; for the R4 time-series: P2, P3, P4, P5, P6, P7, P9, P11, P12, P16, P17 and P19; and for the R5 time-series: P2, P3, P4, P6, P7, P8, P16, P17 and P19

2015-09-24 | E-GEOD-73353 | biostudies-arrayexpress

From mouse to humans: detecting preventing vaccination targets associated to breast cancer stem cells. Exon-level analysis

Project description:TuBo cell line (E) was compared to passage 1 (P1), 2 (P2) and 3 (P3) mammospheres to detect specific transcription isoforms associated to cancer stem cell.

2013-11-30 | GSE21475 | GEO

Discovery of progenitor signatures by time series synexpression analysis during Drosophila cell immortalization [Microarray Expression]

Project description:To characterize the sequence of events associated with RasV12 immortalization of Drosophila embryonic cells, we generated transcriptional time series during cell line establishment, from primary cultures until passage (P) 19. We generated two transcriptional time series from two cell lines (R3 and R7) by sampling the cultures at successive stages, early (P2-4), intermediate (P4-11), and late (P16-19), characterized by different passage times. Time points for the R3 time-series were: P2, P2 (replicate), P5, P6, P7, P8, P16, P17 and P19; for the R7 time-series: P2, P2 (replicate), P3, P4, P7, P8, P16, P17 and P19.

2015-09-23 | E-GEOD-73335 | biostudies-arrayexpress

Analysis of Natural Killer Cell Activity (NKA) in Whole Blood in High Risk Subjects Undergoing Colonoscopy

Project description:This study will measure the activity of natural killer (NK) cells using the in vitro diagnostic device NK Vue in high risk subjects (Quebec risk categories P2, P3 and P4) scheduled for colonoscopy.

| 2251793 | ecrin-mdr-crc

From mouse to humans: detecting preventing vaccination targets associated to breast cancer stem cells. Exon-level analysis

Project description:TuBo cell line (E) was compared to passage 1 (P1), 2 (P2) and 3 (P3) mammospheres to detect specific transcription isoforms associated to cancer stem cell. TuBo cell line (E) was compared to passage 1 (P1), 2 (P2) and 3 (P3) mammospheres. Exon-level transcription profiling was done using Affimetrix GeneChip Exon 1.0 ST. Exon-level analysis is more complex than gene-level analysis. The number of probesets to be investigated is at least 10 times larger in number than gene-level probesets. Thus results, upon statistical analysis, are massively contaminated by type I statistical errors, i.e. false positive [Della Beffa et al. 2008]. Furthermore, exon-level probesets are based only on four probes, which makes exon-level signal summarization more noisy than gene-level, where signal summarization is based on the overall probes encompassed by all exons of a gene. To moderate these issues we have expanded the number of experimental replications: six for TuBo and passage 1 mammosphere, four for passage 3 mammosphere and three for passage 2 mammosphere. Furthermore, after data summarization with RMA and normalization with sketch quantile method, alternative splicing detection between epithelial and mammospheres passages, was assessed using MiDAS, a two way anova developed by Affymetrix for the detection of alternative splicing events. We considered suitable for further investigations the splicing events in common between the sets of exons detected as spliced in each of the following comparisons: E vs P1, E vs P2 and E vs P3.

2013-11-30 | E-GEOD-21475 | biostudies-arrayexpress

Mutation in H82 P0,P1,P2,P3,P4 tumors

Project description:Mutation in H82 P0,P1,P2,P3,P4 tumors

| PRJNA672788 | ENA

Characterization of CD44low CD62Llow CD8 T-cell subset

Project description:To characterize CD44low CD62low CD8 T-cell subset (P4), we compared gene expression among four CD8 T-cell subsets. A hierarchical clustering of global gene expression showed that each subset of CD8 T-cells expresses a distinct gene profile. Genes expressed in activated T-cells were highly expressed in P4 cells compared to naive P1 cells. On the other hand, genes expressed in differentiated T-cells were less expressed in P4 cells compared to either central memory (P2) or effector/memory (P3) cells. These results indicate that P4 cells are in an activated, but pre-mature state and distinct from more differentiated subsets of CD8 T-cells such as central memory and effector/memory cells.

2021-05-17 | GSE161660 | GEO

Transcriptional characterisation of hemopoietic populations in GATA1s ESC-derived Megakarypoieisis

Project description:Embryonic Stem Cells expressing the BirA biotin ligase (BirA) together with a biotinylable version of the full length GATA1 (bioG1) or the truncated GATA1s (bioG1s) transcription factor were differentiated into Embryoid Bodies for 6 days. kithiCD41+ cells were FACS-sorted and grown onto OP9 cells layer for 2 days in the presence of TPO. The kithiCD41lo (P1), kitloCD41lo (P2), kitloCD41hi (P3) and kit-CD41hi (P4) subpopulations were then FACS-sorted and analysed by RNA sequencing

2020-06-15 | E-MTAB-8968 | biostudies-arrayexpress

Proteomic Characterization and Antiproliferative Potential of Proteins from Cuscuta Epithymum Crude Herbal Product Against MCF-7 Human Breast Cancer Cell Line

Project description:Phytochemicals from Cuscuta have been reported for exhibiting anticancer properties; however, the bioactivities attributed to the proteins remained unexplored. This study examined the proteins from a crude herbal product of Cuscuta epithymum (CE) harvested from host plants Alhaji maurorum and Medicago sativa for inhibiting the proliferation of the MCF-7 breast cancer cell line. The CE crude proteins (CP) were separated into four protein peaks (P1, P2, P3, and P4) by gel filtration chromatography. CP, P1, P2, and P3 showed dose-dependent growth inhibitory response in the MTT assay with respective IC50 values of 33.8, 43.1, 34.5, and 28.6 µg/ml. In western-blot analysis, upregulation of BCL2-associated X protein (BAX) with concomitant downregulation of BCL-2 in the cells treated with crude and P3 was observed. In cells treated with P2, BAX was upregulated; however, no significant change in the expression of BCL-2 was recorded. The LC-MS/MS analysis revealed the identity of those proteins in CE, such as peroxidase, non-specific lipid transfer protein, and superoxide dismutase, etc., which have been previously reported from other plants for exhibiting anticancer activity. The findings indicated that Cuscuta proteins strongly inhibited the proliferation of breast cancer MCF-7 cells by apoptosis. The survey of the CE protein profile suggests it as an enriched source of proteins with high biological significance.

2023-08-29 | PXD044708 | Pride

Monitoring of functional gene responses to ERD (enhanced reductive dechlorination) from four TCE-contaminated sites

Project description:A functional microarray targeting 24 genes involved in chlorinated solvent biodegradation pathways has been developed and used to monitor the gene diversity present in four trichloroethylene (TCE) contaminated sites under ERD (enhanced reductive dechlorination) treatment. The microarray format provided by NimbleGen and used in this study is 12x135K. 2 M-BM-5g of labelled gDNA from 30 groundwater samples were hybridized on the microarrays. A 30-chip study was performed, each chip corresponding to hybridization with 2 M-BM-5g of labelled gDNA retrieved from a monitoring well from one of the four contaminated sites. Each probe (760nt) on the microarray was synthesized in eight replicates, and a total of 5,707 random probes was used to determine the background noise. Groundwater samples were collected from four contaminated sites (B, F, G and H), four monitoring wells per site (P1, P2, P3 and P4). P1: well located upstream to the contamination source. P2: well in the contamination source. P3 and P4: wells located downstream to the contamination source. For site B, the monitoring of ERD demonstration was performed through a total of 5 sampling campaigns: C1 (T=0), C2 (T=104 days), C3 (T=231 days), C4 (T=291 days) and C5 (T=378 days). For the three other sites (F, G and H), only one sampling campaign was performed after the treatment.

2012-04-02 | E-GEOD-28606 | biostudies-arrayexpress

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data