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RNA-Seq Analysis of Wild Type and LGMN-/- Peritoneal Macrophage Transcriptomes.

ABSTRACT: Research on the interaction between tumour-associated macrophages (TAMs) and tumour cells has generated great interest in the potential use of macrophages as a therapeutic strategy against tumour cells. Here, we show that deletion of legumain, a conversed proteolytic enzyme in macrophages, promotes senescence of macrophages and tumour cells. Peritoneal macrophages isolated from wide type and lgmn-/- mice were co-cultured with EO771 breast cancer cells for 4 days. Total RNA was extracted for RNA-Seq analysis, cDNA library construction and sequencing were performed using BGISEQ-500 platform.

ORGANISM(S): Mus musculus

PROVIDER: GSE119390 | GEO | 2018/09/04

REPOSITORIES: GEO

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